Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Histone deacetylation promotes transcriptional silencing at facultative heterochromatin

Beth Rosina Watts, Sina Wittmann, Maxime Wery, Camille Gautier, Krzysztof Kus, Dong-Hyuk Heo, Cornelia Kilchert, Antonin Morillon, Lidia Vasiljeva
doi: https://doi.org/10.1101/219535
Beth Rosina Watts
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sina Wittmann
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maxime Wery
2Institute Curie, CNRS UMR 3244, UPMC26 rue d'Ulm, 75248 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Camille Gautier
2Institute Curie, CNRS UMR 3244, UPMC26 rue d'Ulm, 75248 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Krzysztof Kus
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dong-Hyuk Heo
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cornelia Kilchert
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
3Institut für Biochemie, Heinrich-Buff-Ring 17, 35392 Gießen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antonin Morillon
2Institute Curie, CNRS UMR 3244, UPMC26 rue d'Ulm, 75248 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lidia Vasiljeva
1Department of Biochemistry, University of Oxford, OX1 3QU, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lidia.vasilieva@bioch.ox.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

It is important to accurately regulate the expression of genes involved in development and environmental response. In the fission yeast Schizosaccharomyces pombe, meiotic genes are tightly repressed during vegetative growth. Despite being embedded in heterochromatin these genes are transcribed and believed to be repressed primarily at the level of RNA. However, the mechanism of facultative heterochromatin formation and the interplay with transcription regulation is not understood. We show genome-wide that HDAC-dependent histone deacetylation is a major determinant in transcriptional silencing of facultative heterochromatin domains. Indeed, mutation of class I/II HDACs leads to increased transcription of meiotic genes and accumulation of their mRNAs. Mechanistic dissection of the pho1 gene where, in response to phosphate, transient facultative heterochromatin is established by overlapping lncRNA transcription shows that the Clr3 HDAC contributes to silencing independently of SHREC, but in an lncRNA-dependent manner. We propose that HDACs promote facultative heterochromatin by establishing alternative transcriptional silencing.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 14, 2017.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Histone deacetylation promotes transcriptional silencing at facultative heterochromatin
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Histone deacetylation promotes transcriptional silencing at facultative heterochromatin
Beth Rosina Watts, Sina Wittmann, Maxime Wery, Camille Gautier, Krzysztof Kus, Dong-Hyuk Heo, Cornelia Kilchert, Antonin Morillon, Lidia Vasiljeva
bioRxiv 219535; doi: https://doi.org/10.1101/219535
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Histone deacetylation promotes transcriptional silencing at facultative heterochromatin
Beth Rosina Watts, Sina Wittmann, Maxime Wery, Camille Gautier, Krzysztof Kus, Dong-Hyuk Heo, Cornelia Kilchert, Antonin Morillon, Lidia Vasiljeva
bioRxiv 219535; doi: https://doi.org/10.1101/219535

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Molecular Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4658)
  • Biochemistry (10310)
  • Bioengineering (7629)
  • Bioinformatics (26218)
  • Biophysics (13463)
  • Cancer Biology (10638)
  • Cell Biology (15354)
  • Clinical Trials (138)
  • Developmental Biology (8461)
  • Ecology (12768)
  • Epidemiology (2067)
  • Evolutionary Biology (16782)
  • Genetics (11368)
  • Genomics (15418)
  • Immunology (10565)
  • Microbiology (25078)
  • Molecular Biology (10169)
  • Neuroscience (54205)
  • Paleontology (398)
  • Pathology (1659)
  • Pharmacology and Toxicology (2878)
  • Physiology (4320)
  • Plant Biology (9206)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6759)
  • Zoology (1455)