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Embryonic circular RNAs at tandem duplicated genes in zebrafish present new paradigm in gene expression

View ORCID ProfileVanessa Chong-Morrison, Tatjana Sauka-Spengler
doi: https://doi.org/10.1101/220160
Vanessa Chong-Morrison
1University of Oxford, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, Oxford OX3 9DS, United Kingdom
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  • ORCID record for Vanessa Chong-Morrison
Tatjana Sauka-Spengler
1University of Oxford, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, Oxford OX3 9DS, United Kingdom
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  • For correspondence: tatjana.sauka-spengler@imm.ox.ac.uk
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Abstract

Circular RNAs are a puzzling class of RNAs with covalently closed loop structure, lacking 5’ and 3’ ends. Present in all cells, no unifying theme has emerged regarding their function. Here, we use transcriptional data obtained by biotagging in zebrafish to uncover a high-resolution cohort of embryonic circRNAs expressed in nuclear and polysomal subcellular compartments in three developmental cell types. The ample presence of embryonic circRNAs on polysomes contradicts previous reports suggesting predominant nuclear localisation. We uncover a novel class of circRNAs, significantly enriched at tandem duplicated genes. Using newly-developed NGS-based approach, we simultaneously resolve the full sequence of putative "tandem-circRNAs" and their underlying mRNAs. These form by long-range cis-splicing events often between distant tandem duplicated genes, resulting in chimaeric mRNA transcripts and circRNAs containing their supernumerary excised exons, integrated from multiple tandem loci. Taken together, our results suggest that circularisation events rather than circRNAs themselves are functionally important.

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Posted November 15, 2017.
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Embryonic circular RNAs at tandem duplicated genes in zebrafish present new paradigm in gene expression
Vanessa Chong-Morrison, Tatjana Sauka-Spengler
bioRxiv 220160; doi: https://doi.org/10.1101/220160
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Embryonic circular RNAs at tandem duplicated genes in zebrafish present new paradigm in gene expression
Vanessa Chong-Morrison, Tatjana Sauka-Spengler
bioRxiv 220160; doi: https://doi.org/10.1101/220160

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