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Phospho-signal flow from a pole-localized microdomain spatially patterns transcription factor activity

Keren Lasker, Alex von Diezmann, Daniel G. Ahrens, View ORCID ProfileThomas H. Mann, W. E. Moerner, Lucy Shapiro
doi: https://doi.org/10.1101/220293
Keren Lasker
1Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305
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Alex von Diezmann
2Department of Chemistry, Stanford University, Stanford, CA 94305
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Daniel G. Ahrens
1Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305
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Thomas H. Mann
1Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305
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  • ORCID record for Thomas H. Mann
W. E. Moerner
2Department of Chemistry, Stanford University, Stanford, CA 94305
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Lucy Shapiro
1Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305
3Chan Zuckerberg Biohub, San Francisco, CA 94158
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  • For correspondence: shapiro@stanford.edu
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SUMMARY

Selective recruitment and concentration of signaling proteins within membrane-less compartments is a ubiquitous mechanism for subcellular organization. However, little is known about how such dynamic recruitment patterns intracellular signaling and cellular development. Here, we combined transcriptional profiling, reaction-diffusion modeling, and single-molecule tracking to study signal exchange in and out of a microdomain at the cell pole of the asymmetrically dividing bacterium Caulobacter crescentus. Our study revealed that the microdomain is selectively permeable, and that each protein in the signaling pathway that activates the cell fate transcription factor CtrA is sequestered and uniformly concentrated within the microdomain or its proximal membrane. Restricted rates of entry into and escape from the microdomain enhance phospho-signaling, leading to a sublinear gradient of CtrA~P along the long axis of the cell. The spatial patterning of CtrA~P creates a gradient of transcriptional activation that serves to prime asymmetric development of the two daughter cells.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 21, 2018.
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Phospho-signal flow from a pole-localized microdomain spatially patterns transcription factor activity
Keren Lasker, Alex von Diezmann, Daniel G. Ahrens, Thomas H. Mann, W. E. Moerner, Lucy Shapiro
bioRxiv 220293; doi: https://doi.org/10.1101/220293
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Phospho-signal flow from a pole-localized microdomain spatially patterns transcription factor activity
Keren Lasker, Alex von Diezmann, Daniel G. Ahrens, Thomas H. Mann, W. E. Moerner, Lucy Shapiro
bioRxiv 220293; doi: https://doi.org/10.1101/220293

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