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A genome-wide resource for high-throughput genomic tagging of yeast ORFs
Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, View ORCID ProfileAnton Khmelinskii, Emmanuel D Levy, Michael Knop
doi: https://doi.org/10.1101/226811
Matthias Meurer
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Yuanqiang Duan
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Ehud Sass
2Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
Ilia Kats
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Konrad Herbst
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Benjamin C Buchmuller
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Verena Dederer
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Florian Huber
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Daniel Kirrmaier
3Cell Morphogenesis and Signal Transduction, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
Martin Štefl
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Koen Van Laer
4Division of Redox Regulation, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
Tobias P Dick
4Division of Redox Regulation, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
Marius K Lemberg
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Anton Khmelinskii
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
Emmanuel D Levy
2Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
Michael Knop
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
3Cell Morphogenesis and Signal Transduction, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany

Abstract
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae ORFs. It consists of 5661 strains with an acceptor module inserted after each ORF, which can be efficiently replaced with tags or regulatory elements. We validate the library with targeted sequencing and demonstrate its use by tagging the yeast proteome with bright fluorescent proteins, determining how sequences downstream of ORFs influence protein expression and localizing previously undetected proteins.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Posted November 30, 2017.
A genome-wide resource for high-throughput genomic tagging of yeast ORFs
Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, Anton Khmelinskii, Emmanuel D Levy, Michael Knop
bioRxiv 226811; doi: https://doi.org/10.1101/226811
A genome-wide resource for high-throughput genomic tagging of yeast ORFs
Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, Anton Khmelinskii, Emmanuel D Levy, Michael Knop
bioRxiv 226811; doi: https://doi.org/10.1101/226811
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