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A genome-wide resource for high-throughput genomic tagging of yeast ORFs

Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, View ORCID ProfileAnton Khmelinskii, Emmanuel D Levy, Michael Knop
doi: https://doi.org/10.1101/226811
Matthias Meurer
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Yuanqiang Duan
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Ehud Sass
2Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
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Ilia Kats
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Konrad Herbst
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Benjamin C Buchmuller
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Verena Dederer
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Florian Huber
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Daniel Kirrmaier
3Cell Morphogenesis and Signal Transduction, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
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Martin Štefl
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Koen Van Laer
4Division of Redox Regulation, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
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Tobias P Dick
4Division of Redox Regulation, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
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Marius K Lemberg
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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Anton Khmelinskii
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
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  • ORCID record for Anton Khmelinskii
  • For correspondence: m.knop@zmbh.uni-heidelberg.de emmanuel.levy@weizmann.ac.il khmelins.zmbh@gmail.com
Emmanuel D Levy
2Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
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  • For correspondence: m.knop@zmbh.uni-heidelberg.de emmanuel.levy@weizmann.ac.il khmelins.zmbh@gmail.com
Michael Knop
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany
3Cell Morphogenesis and Signal Transduction, DKFZ-ZMBH Alliance and German Cancer Research Center (DKFZ), Heidelberg, Germany
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  • For correspondence: m.knop@zmbh.uni-heidelberg.de emmanuel.levy@weizmann.ac.il khmelins.zmbh@gmail.com
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Abstract

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae ORFs. It consists of 5661 strains with an acceptor module inserted after each ORF, which can be efficiently replaced with tags or regulatory elements. We validate the library with targeted sequencing and demonstrate its use by tagging the yeast proteome with bright fluorescent proteins, determining how sequences downstream of ORFs influence protein expression and localizing previously undetected proteins.

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Posted November 30, 2017.
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A genome-wide resource for high-throughput genomic tagging of yeast ORFs
Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, Anton Khmelinskii, Emmanuel D Levy, Michael Knop
bioRxiv 226811; doi: https://doi.org/10.1101/226811
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A genome-wide resource for high-throughput genomic tagging of yeast ORFs
Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst, Benjamin C Buchmuller, Verena Dederer, Florian Huber, Daniel Kirrmaier, Martin Štefl, Koen Van Laer, Tobias P Dick, Marius K Lemberg, Anton Khmelinskii, Emmanuel D Levy, Michael Knop
bioRxiv 226811; doi: https://doi.org/10.1101/226811

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