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A quantitative PCR based environmental DNA assay for detecting Atlantic salmon (Salmo salar L.)

Siobhán Atkinson, Jeannette E.L. Carlsson, Bernard Ball, Damian Egan, Mary Kelly-Quinn, Ken Whelan, Jens Carlsson
doi: https://doi.org/10.1101/226829
Siobhán Atkinson
1School of Biology & Environmental Science, University College Dublin, Dublin, Ireland
2Area52 Research Group, School of Biology & Environmental Science/Earth Institute, University College Dublin, Dublin, Ireland
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  • For correspondence: siobhan.atkinson@ucdconnect.ie
Jeannette E.L. Carlsson
2Area52 Research Group, School of Biology & Environmental Science/Earth Institute, University College Dublin, Dublin, Ireland
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Bernard Ball
2Area52 Research Group, School of Biology & Environmental Science/Earth Institute, University College Dublin, Dublin, Ireland
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Damian Egan
1School of Biology & Environmental Science, University College Dublin, Dublin, Ireland
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Mary Kelly-Quinn
1School of Biology & Environmental Science, University College Dublin, Dublin, Ireland
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Ken Whelan
1School of Biology & Environmental Science, University College Dublin, Dublin, Ireland
3Atlantic Salmon Trust, 11 Rutland Square, Edinburgh, Scotland
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Jens Carlsson
2Area52 Research Group, School of Biology & Environmental Science/Earth Institute, University College Dublin, Dublin, Ireland
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Abstract

  1. The Atlantic salmon (Salmo salar L.) has worldwide ecological, cultural and economic importance. The species has undergone extensive decline across its native range, yet concerns have been raised about its invasive potential in the Pacific. Knowledge on the distribution of this species is vital for addressing conservation goals.

  2. This study presents an eDNA assay to detect S. salar in water samples, using quantitative PCR (qPCR) technology. Species-specific primers and a minor groove binding (MGB) probe were designed for the assay, based on the mitochondrial cytochrome oxidase I (COI) gene.

  3. The results of this study indicate that eDNA is a highly sensitive tool for detecting S. salar in situ, and could potentially provide an alternative, non-invasive method for determining the distribution of this species.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 29, 2017.
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A quantitative PCR based environmental DNA assay for detecting Atlantic salmon (Salmo salar L.)
Siobhán Atkinson, Jeannette E.L. Carlsson, Bernard Ball, Damian Egan, Mary Kelly-Quinn, Ken Whelan, Jens Carlsson
bioRxiv 226829; doi: https://doi.org/10.1101/226829
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A quantitative PCR based environmental DNA assay for detecting Atlantic salmon (Salmo salar L.)
Siobhán Atkinson, Jeannette E.L. Carlsson, Bernard Ball, Damian Egan, Mary Kelly-Quinn, Ken Whelan, Jens Carlsson
bioRxiv 226829; doi: https://doi.org/10.1101/226829

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