Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Actin Redundancy in Chlamydomonas is Required for Flagellar Protein Synthesis and Gating

Brittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, View ORCID ProfilePrachee Avasthi
doi: https://doi.org/10.1101/227553
Brittany Jack
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David M. Mueller
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ann C. Fee
2University of Missouri-Kansas City, School of Medicine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashley Tetlow
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Prachee Avasthi
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
3Department of Ophthalmology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Prachee Avasthi
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Flagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of mammalian cells and provide an excellent model to study ciliogenesis. These biflagellated cells have two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Previously, we described a role for actin in regulation of flagella-building intraflagellar transport machinery and now probe how actin redundancy contributes to this process using a nap1 mutant Chlamydomonas strain. Treatment with Latrunculin B, a potent actin polymerization inhibitor on the nap1 mutant background acutely disrupts all filamentous actins in the cell. We find that actins are an absolute requirement for flagellar growth when the preexisting pool of flagellar precursors is depleted. Nap1 mutants treated with Latrunculin B also showed reduced protein synthesis during regeneration. Finally, loss of functional actins reduced the incorporation of existing flagellar proteins as well as caused mislocalization of a key transition zone gating protein, NPHP-4. These experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for actin in incorporation of newly synthesized flagellar proteins.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted November 30, 2017.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Actin Redundancy in Chlamydomonas is Required for Flagellar Protein Synthesis and Gating
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Actin Redundancy in Chlamydomonas is Required for Flagellar Protein Synthesis and Gating
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, Prachee Avasthi
bioRxiv 227553; doi: https://doi.org/10.1101/227553
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Actin Redundancy in Chlamydomonas is Required for Flagellar Protein Synthesis and Gating
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, Prachee Avasthi
bioRxiv 227553; doi: https://doi.org/10.1101/227553

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2633)
  • Biochemistry (5221)
  • Bioengineering (3643)
  • Bioinformatics (15711)
  • Biophysics (7213)
  • Cancer Biology (5593)
  • Cell Biology (8045)
  • Clinical Trials (138)
  • Developmental Biology (4735)
  • Ecology (7462)
  • Epidemiology (2059)
  • Evolutionary Biology (10520)
  • Genetics (7698)
  • Genomics (10082)
  • Immunology (5148)
  • Microbiology (13823)
  • Molecular Biology (5354)
  • Neuroscience (30577)
  • Paleontology (211)
  • Pathology (871)
  • Pharmacology and Toxicology (1519)
  • Physiology (2234)
  • Plant Biology (4983)
  • Scientific Communication and Education (1036)
  • Synthetic Biology (1379)
  • Systems Biology (4130)
  • Zoology (803)