Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization

View ORCID ProfileBrittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, View ORCID ProfilePrachee Avasthi
doi: https://doi.org/10.1101/227553
Brittany Jack
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Brittany Jack
David M. Mueller
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ann C. Fee
2University of Missouri-Kansas City, School of Medicine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashley Tetlow
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Prachee Avasthi
1Department of Anatomy and Cell-Biology University of Kansas Medical Center
3Department of Ophthalmology University of Kansas Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Prachee Avasthi
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Flagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of mammalian cells and provide an excellent model to study ciliogenesis. These biflagellated cells have two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Previously, we described a role for actin in the regulation of flagella-building intraflagellar transport machinery. Here, we probe how actin redundancy contributes to this process using a nap1 mutant Chlamydomonas strain. Disruption of a single actin allows normal or slower incorporation but complete flagellar assembly. However, when we disrupt both actins using Latrunculin B (LatB) treatment on the nap1 mutant background, we find flagellar growth from newly synthesized limiting flagellar proteins is actin-dependent. Upon total actin disruption during flagellar assembly, transmission electron microscopy identified an accumulation of Golgi-adjacent vesicles, suggesting impaired vesicular trafficking may be the mechanism by which actin supports flagellar growth from new flagellar proteins. We also find there is a mislocalization of a key transition zone gating and ciliopathy protein, NPHP-4. Extended (2 hour) treatment with LatB, a condition under which NAP1 is upregulated, restores NPHP-4 localization. This suggests NAP1 can perform the functions of conventional actin at the transition zone. Our experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for these actins in transport of Golgi-adjacent vesicles and flagellar incorporation of newly synthesized proteins.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 03, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, Prachee Avasthi
bioRxiv 227553; doi: https://doi.org/10.1101/227553
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley Tetlow, Prachee Avasthi
bioRxiv 227553; doi: https://doi.org/10.1101/227553

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4246)
  • Biochemistry (9176)
  • Bioengineering (6807)
  • Bioinformatics (24066)
  • Biophysics (12160)
  • Cancer Biology (9567)
  • Cell Biology (13847)
  • Clinical Trials (138)
  • Developmental Biology (7661)
  • Ecology (11739)
  • Epidemiology (2066)
  • Evolutionary Biology (15547)
  • Genetics (10673)
  • Genomics (14366)
  • Immunology (9515)
  • Microbiology (22916)
  • Molecular Biology (9135)
  • Neuroscience (49170)
  • Paleontology (358)
  • Pathology (1487)
  • Pharmacology and Toxicology (2584)
  • Physiology (3851)
  • Plant Biology (8351)
  • Scientific Communication and Education (1473)
  • Synthetic Biology (2301)
  • Systems Biology (6207)
  • Zoology (1304)