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Combined aptamer and transcriptome sequencing of single cells

View ORCID ProfileCyrille L. Delley, Leqian Liu, Maen F. Sarhan, Adam R. Abate
doi: https://doi.org/10.1101/228338
Cyrille L. Delley
1Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158 California, USA
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  • ORCID record for Cyrille L. Delley
Leqian Liu
1Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158 California, USA
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Maen F. Sarhan
1Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158 California, USA
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Adam R. Abate
1Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158 California, USA
2California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, 94158 California, USA
3Chan Zuckerberg Biohub, San Francisco, 94158 California, USA.
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  • For correspondence: adam@abatelab.org
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Abstract

The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.

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Posted December 03, 2017.
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Combined aptamer and transcriptome sequencing of single cells
Cyrille L. Delley, Leqian Liu, Maen F. Sarhan, Adam R. Abate
bioRxiv 228338; doi: https://doi.org/10.1101/228338
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Combined aptamer and transcriptome sequencing of single cells
Cyrille L. Delley, Leqian Liu, Maen F. Sarhan, Adam R. Abate
bioRxiv 228338; doi: https://doi.org/10.1101/228338

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