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Spotiton: New Features and Applications

Venkata P. Dandey, Hui Wei, Zhening Zhang, Yong Zi Tan, Priyamvada Acharya, Edward T. Eng, William J. Rice, Peter A. Kahn, Clinton S. Potter, Bridget Carragher
doi: https://doi.org/10.1101/230151
Venkata P. Dandey
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
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Hui Wei
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
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Zhening Zhang
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
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Yong Zi Tan
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
2Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
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Priyamvada Acharya
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
3Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Maryland
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Edward T. Eng
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
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William J. Rice
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
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Peter A. Kahn
4Engineering Arts, Arizona.
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Clinton S. Potter
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
2Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
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Bridget Carragher
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA.
2Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
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Abstract

We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ~3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 08, 2017.
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Spotiton: New Features and Applications
Venkata P. Dandey, Hui Wei, Zhening Zhang, Yong Zi Tan, Priyamvada Acharya, Edward T. Eng, William J. Rice, Peter A. Kahn, Clinton S. Potter, Bridget Carragher
bioRxiv 230151; doi: https://doi.org/10.1101/230151
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Spotiton: New Features and Applications
Venkata P. Dandey, Hui Wei, Zhening Zhang, Yong Zi Tan, Priyamvada Acharya, Edward T. Eng, William J. Rice, Peter A. Kahn, Clinton S. Potter, Bridget Carragher
bioRxiv 230151; doi: https://doi.org/10.1101/230151

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