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Isolation of nucleic acids from low biomass samples: detection and removal of sRNA contaminants

View ORCID ProfileAnna Heintz-Buschart, View ORCID ProfileDilmurat Yusuf, Anne Kaysen, Alton Etheridge, Joёlle V. Fritz, View ORCID ProfilePatrick May, Carine de Beaufort, Bimal B. Upadhyaya, Anubrata Ghosal, David J Galas, View ORCID ProfilePaul Wilmes
doi: https://doi.org/10.1101/232975
Anna Heintz-Buschart
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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  • ORCID record for Anna Heintz-Buschart
  • For correspondence: paul.wilmes@uni.lu anna.heintz-buschart@idiv.de
Dilmurat Yusuf
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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Anne Kaysen
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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Alton Etheridge
2Pacific Northwest Research Institute, Seattle, WA, 98122, USA
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Joёlle V. Fritz
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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Patrick May
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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Carine de Beaufort
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
3Centre Hospitalier de Luxembourg, 1210 Luxembourg, Luxembourg
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Bimal B. Upadhyaya
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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Anubrata Ghosal
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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David J Galas
2Pacific Northwest Research Institute, Seattle, WA, 98122, USA
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Paul Wilmes
1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
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  • ORCID record for Paul Wilmes
  • For correspondence: paul.wilmes@uni.lu anna.heintz-buschart@idiv.de
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Article Information

doi 
https://doi.org/10.1101/232975
History 
  • December 14, 2017.

Article Versions

  • You are currently viewing Version 1 of this article (December 14, 2017 - 10:52).
  • Version 2 (December 20, 2017 - 12:59).
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Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.

Author Information

  1. Anna Heintz-Buschart1,*,
  2. Dilmurat Yusuf1,
  3. Anne Kaysen1,
  4. Alton Etheridge2,
  5. Joёlle V. Fritz1,
  6. Patrick May1,
  7. Carine de Beaufort1,3,
  8. Bimal B. Upadhyaya1,
  9. Anubrata Ghosal1,
  10. David J Galas2 and
  11. Paul Wilmes1,*
  1. 1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 4362 Esch-sur-Alzette, Luxembourg
  2. 2Pacific Northwest Research Institute, Seattle, WA, 98122, USA
  3. 3Centre Hospitalier de Luxembourg, 1210 Luxembourg, Luxembourg
  1. ↵* To whom correspondence should be addressed. Paul Wilmes; Tel: 00352-466644-6188; Fax: 00352466644-6949; Email: paul.wilmes{at}uni.lu; correspondence may also be addressed to Anna Heintz-Buschart; Tel: 0049-345-558-5225; Email: anna.heintz-buschart{at}idiv.de
  • Present Addresses:

  • Anna Heintz-Buschart, German Centre for Integrative Biodiversity Research (iDiv) Leipzig-Halle-Jena, 04103 Leipzig, Germany, and Department of Soil Ecology, Helmholtz-Centre for Environmental Research GmbH (UFZ), 06120 Halle (Saale), Germany

  • Anne Kaysen, Centre Hospitalier de Luxembourg, 1210 Luxembourg, Luxembourg

  • Joёlle V. Fritz, Centre Hospitalier de Luxembourg, 1210 Luxembourg, Luxembourg

  • Dilmurat Yusuf, Bioinformatics Group, Department of Computer Science, University of Freiburg, Freiburg, 79110, Germany

  • Anubrata Ghosal, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States

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Isolation of nucleic acids from low biomass samples: detection and removal of sRNA contaminants
Anna Heintz-Buschart, Dilmurat Yusuf, Anne Kaysen, Alton Etheridge, Joёlle V. Fritz, Patrick May, Carine de Beaufort, Bimal B. Upadhyaya, Anubrata Ghosal, David J Galas, Paul Wilmes
bioRxiv 232975; doi: https://doi.org/10.1101/232975
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Isolation of nucleic acids from low biomass samples: detection and removal of sRNA contaminants
Anna Heintz-Buschart, Dilmurat Yusuf, Anne Kaysen, Alton Etheridge, Joёlle V. Fritz, Patrick May, Carine de Beaufort, Bimal B. Upadhyaya, Anubrata Ghosal, David J Galas, Paul Wilmes
bioRxiv 232975; doi: https://doi.org/10.1101/232975

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