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An unconventional NOI/RIN4 domain of a rice NLR protein binds host EXO70 protein to confer fungal immunity

Koki Fujisaki, Yoshiko Abe, Eiko Kanzaki, Kazue Ito, Hiroe Utsushi, Hiromasa Saitoh, Aleksandra Białas, Mark J Banfield, Sophien Kamoun, Ryohei Terauchi
doi: https://doi.org/10.1101/239400
Koki Fujisaki
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
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Yoshiko Abe
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
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Eiko Kanzaki
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
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Kazue Ito
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
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Hiroe Utsushi
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
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Hiromasa Saitoh
2Department of Molecular Microbiology, Tokyo University of Agriculture, Setagaya, Tokyo, Japan,
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Aleksandra Białas
3The Sainsbury Laboratory, Norwich Research Park, Norwich, UK,
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Mark J Banfield
3The Sainsbury Laboratory, Norwich Research Park, Norwich, UK,
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Sophien Kamoun
3The Sainsbury Laboratory, Norwich Research Park, Norwich, UK,
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Ryohei Terauchi
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan,
5Laboratory of Crop Evolution, Kyoto University, Muko, Kyoto, Japan
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Abstract

A subset of plant nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins carry extraneous integrated domains that have been proposed to mediate pathogen effector recognition. The current view is that these unconventional domains function by directly binding or serving as substrates for pathogen effectors, yet only a few domains have been functionally characterized to date. Here we report that the integrated NOI domain of the rice NLR protein Pii-2, together with its partner Pii-1, mediates immunity to the rice blast fungus Magnaporthe oryzae by indirect recognition of the AVR-Pii effector. We discovered that the Pii-2 NOI domain does not physically interact with the effector itself but instead binds the host protein OsExo70-F3, which is a target of AVR-Pii. We further identified mutations within the NOI core motif (PxFGxW) of Pii-2 that abolish both OsExo70-F3 binding and Pii-mediated resistance to M. oryzae expressing AVR-Pii. This led us to propose a novel conceptual model in which an NLR-integrated domain functions to detect host proteins targeted by pathogen effectors, in a framework that extends classical indirect recognition models.

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Posted December 24, 2017.
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An unconventional NOI/RIN4 domain of a rice NLR protein binds host EXO70 protein to confer fungal immunity
Koki Fujisaki, Yoshiko Abe, Eiko Kanzaki, Kazue Ito, Hiroe Utsushi, Hiromasa Saitoh, Aleksandra Białas, Mark J Banfield, Sophien Kamoun, Ryohei Terauchi
bioRxiv 239400; doi: https://doi.org/10.1101/239400
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An unconventional NOI/RIN4 domain of a rice NLR protein binds host EXO70 protein to confer fungal immunity
Koki Fujisaki, Yoshiko Abe, Eiko Kanzaki, Kazue Ito, Hiroe Utsushi, Hiromasa Saitoh, Aleksandra Białas, Mark J Banfield, Sophien Kamoun, Ryohei Terauchi
bioRxiv 239400; doi: https://doi.org/10.1101/239400

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