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3.7 Å cryo-EM structure of the core Centromere Binding Factor 3 complex

Wenjuan Zhang, Natalya Lukoynova, Shomon Miah, View ORCID ProfileCara K Vaughan
doi: https://doi.org/10.1101/241067
Wenjuan Zhang
Birkbeck College;
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Natalya Lukoynova
Birkbeck College;
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Shomon Miah
Birkbeck College;
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Cara K Vaughan
Institute of Structural and Molecular Biology
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  • ORCID record for Cara K Vaughan
  • For correspondence: c.vaughan@mail.cryst.bbk.ac.uk
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Abstract

The Centromere Binding Factor 3 (CBF3) complex binds the third Centromere DNA Element in organisms with point centromeres, such as S. cerevisiae. It is the only essential centromere binding complex as it facilitates genetic specification of point centromeres. It is therefore the most fundamental complex of the kinetochore in these organisms and its association with centromere DNA allows association of all other kinetochore components. We have determined the atomic structure of the core complex of CBF3, comprising 3 of its 4 components, using cryo-EM. The architecture of the complex is U-shaped, with a deep, strongly basic channel that is narrow at one end and wide at the other. Combining our structure and in vitro assays, we present a model for its association with centromere DNA.

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Posted December 29, 2017.
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3.7 Å cryo-EM structure of the core Centromere Binding Factor 3 complex
Wenjuan Zhang, Natalya Lukoynova, Shomon Miah, Cara K Vaughan
bioRxiv 241067; doi: https://doi.org/10.1101/241067
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3.7 Å cryo-EM structure of the core Centromere Binding Factor 3 complex
Wenjuan Zhang, Natalya Lukoynova, Shomon Miah, Cara K Vaughan
bioRxiv 241067; doi: https://doi.org/10.1101/241067

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