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Dynamin-2 facilitates Atg9 recycling from nascent autophagosomes

Alejandro Martorell Riera, Cinta Iriondo Martinez, Samuel Itskanov, Janos Steffen, Brett Roach, Carla M. Koehler, View ORCID ProfileAlexander M. van der Bliek
doi: https://doi.org/10.1101/241901
Alejandro Martorell Riera
1Department of Biological Chemistry, David Geffen School of medicine at UCLA
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Cinta Iriondo Martinez
1Department of Biological Chemistry, David Geffen School of medicine at UCLA
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Samuel Itskanov
1Department of Biological Chemistry, David Geffen School of medicine at UCLA
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Janos Steffen
2Department of Chemistry and Biochemistry, UCLA
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Brett Roach
1Department of Biological Chemistry, David Geffen School of medicine at UCLA
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Carla M. Koehler
2Department of Chemistry and Biochemistry, UCLA
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Alexander M. van der Bliek
1Department of Biological Chemistry, David Geffen School of medicine at UCLA
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  • ORCID record for Alexander M. van der Bliek
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Abstract

Autophagy involves rapid growth of phagophores through membrane addition. Newly added membranes are derived from other organelles through the formation of vesicles carrying the Atg9 protein. Membrane is delivered by fusing these vesicles with the phagophores. Atg9 is, nevertheless, not incorporated in autophagosomes. We now show that this protein is retrieved from phagophores by fission utilizing Dynamin-2 (Dnm2) as the membrane scission protein. Blocking Atg9 recycling by interfering with Dnm2 helps retain Atg9 in autophagosomes and degrades this protein by autophagy. Dnm2 colocalizes with the BAR domain protein Endophilin-B1 (EndoB1/Bif-1) when autophagy is induced, suggestive of transient interactions, but mutations in these proteins have very different effects from which we conclude that they act at different stages of autophagy. Our results show that Dnm2 promotes Atg9 retrieval, while EndoB1 may anchor the autophagy machinery. These data provide novel insights into the roles of membrane scission proteins during autophagy.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 06, 2018.
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Dynamin-2 facilitates Atg9 recycling from nascent autophagosomes
Alejandro Martorell Riera, Cinta Iriondo Martinez, Samuel Itskanov, Janos Steffen, Brett Roach, Carla M. Koehler, Alexander M. van der Bliek
bioRxiv 241901; doi: https://doi.org/10.1101/241901
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Dynamin-2 facilitates Atg9 recycling from nascent autophagosomes
Alejandro Martorell Riera, Cinta Iriondo Martinez, Samuel Itskanov, Janos Steffen, Brett Roach, Carla M. Koehler, Alexander M. van der Bliek
bioRxiv 241901; doi: https://doi.org/10.1101/241901

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