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The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization

View ORCID ProfilePedro Prudêncio, View ORCID ProfileLeonardo G. Guilgur, View ORCID ProfileJoão Sobral, View ORCID ProfileJörg D. Becker, View ORCID ProfileRui Gonçalo Martinho, View ORCID ProfilePaulo Navarro-Costa
doi: https://doi.org/10.1101/242008
Pedro Prudêncio
2Center for Biomedical Research, Universidade do Algarve. Faro, Portugal.
3Instituto de Medicina Molecular. Lisboa, Portugal.
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Leonardo G. Guilgur
1Instituto Gulbenkian de Ciência. Oeiras, Portugal.
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  • ORCID record for Leonardo G. Guilgur
João Sobral
1Instituto Gulbenkian de Ciência. Oeiras, Portugal.
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Jörg D. Becker
1Instituto Gulbenkian de Ciência. Oeiras, Portugal.
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Rui Gonçalo Martinho
2Center for Biomedical Research, Universidade do Algarve. Faro, Portugal.
3Instituto de Medicina Molecular. Lisboa, Portugal.
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  • For correspondence: rgmartinho@ualg.pt navarro-costa@medicina.ulisboa.pt
Paulo Navarro-Costa
1Instituto Gulbenkian de Ciência. Oeiras, Portugal.
2Center for Biomedical Research, Universidade do Algarve. Faro, Portugal.
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  • For correspondence: rgmartinho@ualg.pt navarro-costa@medicina.ulisboa.pt
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ABSTRACT

The transition from fertilized oocyte to totipotent embryo relies on maternally-provided factors that are synthetized and accumulated in developing oocytes. Yet, it is still unclear how oocytes regulate the expression of these embryo fate-promoting genes within the general transcriptional program of oogenesis. Here we report that the Drosophila Trithorax group protein MLL3/4 (dMLL3/4, also known as Trr) is essential for the transition to embryo fate at fertilization. In the absence of dMLL3/4, oocytes develop normally but fail to initiate the embryo mitotic divisions after fertilization. This incapability results from defects in both paternal genome reprogramming and maternal meiotic completion. We show that, during oogenesis, dMLL3/4 promotes the expression of a functionally coherent gene subset that is later required for the correct assembly of the zygotic genome. Accordingly, we identify the evolutionarily-conserved IDGF4 glycoprotein (known as oviductin in mammals) as a new oocyte-to-embryo transition gene under dMLL3/4 transcriptional control. Based on these observations, we propose that dMLL3/4 plays an instructive role in the oocyte-to-embryo transition that is functionally uncoupled from the requirements of normal oocyte differentiation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 04, 2018.
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The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization
Pedro Prudêncio, Leonardo G. Guilgur, João Sobral, Jörg D. Becker, Rui Gonçalo Martinho, Paulo Navarro-Costa
bioRxiv 242008; doi: https://doi.org/10.1101/242008
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The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization
Pedro Prudêncio, Leonardo G. Guilgur, João Sobral, Jörg D. Becker, Rui Gonçalo Martinho, Paulo Navarro-Costa
bioRxiv 242008; doi: https://doi.org/10.1101/242008

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