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A Barley Powdery Mildew Fungus Non-Autonomous Retrotransposon Encodes a Peptide that Supports Penetration Success on Barley

Mathias Nottensteiner, Bernd Zechmann, Christopher McCollum, View ORCID ProfileRalph Hückelhoven
doi: https://doi.org/10.1101/242271
Mathias Nottensteiner
1Chair of Phytopathology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany
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Bernd Zechmann
2Center for Microscopy and Imaging, Baylor University, Waco, TX 76798-7046, USA
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Christopher McCollum
1Chair of Phytopathology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany
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Ralph Hückelhoven
1Chair of Phytopathology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany
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  • ORCID record for Ralph Hückelhoven
  • For correspondence: hueckelhoven@wzw.tum.de
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ABSTRACT

Plant immunity is overcome by pathogens by the means of secreted effectors. Host effector targets might be proteins acting in pathogen defense or serve demands of the pathogen. The barley ROP GTPase HvRACB is involved in entry of the powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh) into barley epidermal cells. We found that HvRACB interacts with the ROP-interactive peptide 1 (ROPIP1) that is encoded on the active non-long terminal repeat retroelement Eg-R1 of Bgh. Over-expression of ROPIP1 in barley epidermal cells and host-induced post-transcriptional gene silencing (HIGS) of ROPIP1 suggested that ROPIP1 is involved in virulence of Bgh. Bimolecular fluorescence complementation and co-localization supported that ROPIP1 can interact with activated HvRACB in planta. We show that ROPIP1 is expressed by Bgh on barley and translocated into the cytoplasm of infected barley cells. ROPIP1 is recruited to microtubules upon co-expression of MICROTUBULE ASSOCIATED ROP GTPase ACTIVATING PROTEIN (HvMAGAP1) and can destabilize cortical microtubules. Bgh ROPIP might target HvRACB and manipulate host cell microtubule organization for facilitated host cell entry. Data suggest a possible neo-functionalization of retroelement-derived transcripts for the evolution of a pathogen virulence effector.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 03, 2018.
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A Barley Powdery Mildew Fungus Non-Autonomous Retrotransposon Encodes a Peptide that Supports Penetration Success on Barley
Mathias Nottensteiner, Bernd Zechmann, Christopher McCollum, Ralph Hückelhoven
bioRxiv 242271; doi: https://doi.org/10.1101/242271
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A Barley Powdery Mildew Fungus Non-Autonomous Retrotransposon Encodes a Peptide that Supports Penetration Success on Barley
Mathias Nottensteiner, Bernd Zechmann, Christopher McCollum, Ralph Hückelhoven
bioRxiv 242271; doi: https://doi.org/10.1101/242271

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