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Viral gain-of-function experiments uncover residues under diversifying selection in nature

Rohan Maddamsetti, Daniel T. Johnson, Stephanie J. Spielman, Katherine L. Petrie, Debora S. Marks, Justin R. Meyer
doi: https://doi.org/10.1101/242495
Rohan Maddamsetti
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
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Daniel T. Johnson
2Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA
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Stephanie J. Spielman
3Institute for Genomics and Evolutionary Medicine, Temple University, Philadelphia, PA, USA
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Katherine L. Petrie
2Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA
4Earth-Life Science Institute, Tokyo Institute of Technology, Japan
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Debora S. Marks
1Department of Systems Biology, Harvard Medical School, Boston, MA, USA
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Justin R. Meyer
2Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA
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Abstract

Viral gain-of-function mutations are commonly observed in the laboratory; however, it is unknown whether those mutations also evolve in nature. We identify two key residues in the host recognition protein of bacteriophage λ that are necessary to exploit a new receptor; both residues repeatedly evolved among homologs from environmental samples. Our results provide evidence for widespread host-shift evolution in nature and a proof of concept for integrating experiments with genomic epidemiology.

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Posted January 03, 2018.
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Viral gain-of-function experiments uncover residues under diversifying selection in nature
Rohan Maddamsetti, Daniel T. Johnson, Stephanie J. Spielman, Katherine L. Petrie, Debora S. Marks, Justin R. Meyer
bioRxiv 242495; doi: https://doi.org/10.1101/242495
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Viral gain-of-function experiments uncover residues under diversifying selection in nature
Rohan Maddamsetti, Daniel T. Johnson, Stephanie J. Spielman, Katherine L. Petrie, Debora S. Marks, Justin R. Meyer
bioRxiv 242495; doi: https://doi.org/10.1101/242495

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