Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration

Rosemary C Challis, Sripriya Ravindra Kumar, Ken Y Chan, Collin Challis, Min J Jang, Pradeep S Rajendran, John D Tompkins, Kalyanam Shivkumar, Benjamin E Deverman, Viviana Gradinaru
doi: https://doi.org/10.1101/246405
Rosemary C Challis
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sripriya Ravindra Kumar
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ken Y Chan
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Collin Challis
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Min J Jang
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pradeep S Rajendran
2Cardiac Arrhythmia Center and Neurocardiology Research Center of Excellence, University of California, Los Angeles Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John D Tompkins
2Cardiac Arrhythmia Center and Neurocardiology Research Center of Excellence, University of California, Los Angeles Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kalyanam Shivkumar
2Cardiac Arrhythmia Center and Neurocardiology Research Center of Excellence, University of California, Los Angeles Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin E Deverman
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Viviana Gradinaru
1Division of Biology and Biological Engineering, California Institute of Technology Pasadena, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: viviana@caltech.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

We recently developed novel AAV capsids for efficient and noninvasive gene transfer across the central and peripheral nervous systems. In this protocol, we describe how to produce and systemically administer AAV-PHP viruses to label and/or genetically manipulate cells in the mouse nervous system and organs including the heart. The procedure comprises three separate stages: AAV production, intravenous delivery, and evaluation of transgene expression. The protocol spans eight days, excluding the time required to assess gene expression, and can be readily adopted by laboratories with standard molecular and cell culture capabilities. We provide guidelines for experimental design and choosing the capsid, cargo, and viral dose appropriate for the experimental aims. The procedures outlined here are adaptable to diverse biomedical applications, from anatomical and functional mapping to gene expression, silencing, and editing.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 19, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration
Rosemary C Challis, Sripriya Ravindra Kumar, Ken Y Chan, Collin Challis, Min J Jang, Pradeep S Rajendran, John D Tompkins, Kalyanam Shivkumar, Benjamin E Deverman, Viviana Gradinaru
bioRxiv 246405; doi: https://doi.org/10.1101/246405
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration
Rosemary C Challis, Sripriya Ravindra Kumar, Ken Y Chan, Collin Challis, Min J Jang, Pradeep S Rajendran, John D Tompkins, Kalyanam Shivkumar, Benjamin E Deverman, Viviana Gradinaru
bioRxiv 246405; doi: https://doi.org/10.1101/246405

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (2524)
  • Biochemistry (4971)
  • Bioengineering (3478)
  • Bioinformatics (15198)
  • Biophysics (6890)
  • Cancer Biology (5385)
  • Cell Biology (7727)
  • Clinical Trials (138)
  • Developmental Biology (4525)
  • Ecology (7143)
  • Epidemiology (2059)
  • Evolutionary Biology (10217)
  • Genetics (7507)
  • Genomics (9776)
  • Immunology (4835)
  • Microbiology (13197)
  • Molecular Biology (5136)
  • Neuroscience (29405)
  • Paleontology (203)
  • Pathology (836)
  • Pharmacology and Toxicology (1462)
  • Physiology (2134)
  • Plant Biology (4739)
  • Scientific Communication and Education (1008)
  • Synthetic Biology (1338)
  • Systems Biology (4008)
  • Zoology (768)