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Resistance gene discovery and cloning by sequence capture and association genetics

Sanu Arora, Burkhard Steuernagel, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D. G. Jones, Alison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, View ORCID ProfileBrande B. H. Wulff
doi: https://doi.org/10.1101/248146
Sanu Arora
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Burkhard Steuernagel
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Sutha Chandramohan
2Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT 2601, Australia
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Yunming Long
3Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA
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Oadi Matny
4Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, USA
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Ryan Johnson
4Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, USA
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Jacob Enk
5Arbor Biosciences, 5840 Interface Dr, Suite 101, Ann Arbor, MI 48103, USA
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Sambasivam Periyannan
2Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT 2601, Australia
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M. Asyraf Md Hatta
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
6Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
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Naveenkumar Athiyannan
2Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT 2601, Australia
7Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia
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Jitender Cheema
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Guotai Yu
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Ngonidzashe Kangara
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Sreya Ghosh
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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Les J. Szabo
8U. S. Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, Minnesota 55108, USA
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Jesse Poland
9Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan KS, USA
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Harbans Bariana
10The University of Sydney, Plant Breeding Institute, 107 Cobbitty Road, Cobbity, NSW, Australia
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Jonathan D. G. Jones
11The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, UK
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Alison R. Bentley
12The John Bingham Laboratory, NIAB, Huntingdon Road, Cambridge CB3 0LE, UK
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Mick Ayliffe
2Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT 2601, Australia
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Eric Olson
13Michigan State University, East Lansing, MI 48824, USA
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Steven S. Xu
14U. S. Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA
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Brian J. Steffenson
4Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, USA
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Evans Lagudah
2Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT 2601, Australia
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Brande B. H. Wulff
1John Innes Centre, NR4 7UH, Norwich Research Park, Norwich, NR4 7UH, UK
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  • ORCID record for Brande B. H. Wulff
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Abstract

Genetic resistance is the most economic and environmentally sustainable approach for crop disease protection. Disease resistance (R) genes from wild relatives are a valuable resource for breeding resistant crops. However, introgression of R genes into crops is a lengthy process often associated with co-integration of deleterious linked genes1, 2 and pathogens can rapidly evolve to overcome R genes when deployed singly3. Introducing multiple cloned R genes into crops as a stack would avoid linkage drag and delay emergence of resistance-breaking pathogen races4. However, current R gene cloning methods require segregating or mutant progenies5–10, which are difficult to generate for many wild relatives due to poor agronomic traits. We exploited natural pan-genome variation in a wild diploid wheat by combining association genetics with R gene enrichment sequencing (AgRenSeq) to clone four stem rust resistance genes in <6 months. RenSeq combined with diversity panels is therefore a major advance in isolating R genes for engineering broad-spectrum resistance in crops.

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Posted January 15, 2018.
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Resistance gene discovery and cloning by sequence capture and association genetics
Sanu Arora, Burkhard Steuernagel, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D. G. Jones, Alison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B. H. Wulff
bioRxiv 248146; doi: https://doi.org/10.1101/248146
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Resistance gene discovery and cloning by sequence capture and association genetics
Sanu Arora, Burkhard Steuernagel, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse Poland, Harbans Bariana, Jonathan D. G. Jones, Alison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B. H. Wulff
bioRxiv 248146; doi: https://doi.org/10.1101/248146

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