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Structure of the Core of the Type Three Secretion System Export Apparatus

Lucas Kuhlen, Patrizia Abrusci, Steven Johnson, Joseph Gault, Justin Deme, Joseph Caesar, Tobias Dietsche, Mehari Tesfazgi Mebrhatu, Tariq Ganief, Boris Macek, Samuel Wagner, Carol V. Robinson, View ORCID ProfileSusan M. Lea
doi: https://doi.org/10.1101/249128
Lucas Kuhlen
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
2Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK.
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Patrizia Abrusci
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
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Steven Johnson
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
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Joseph Gault
2Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK.
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Justin Deme
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
3Central Oxford Structural Microscopy and Imaging Centre, University of Oxford, South Parks Road, Oxford OX1 3RE.
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Joseph Caesar
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
3Central Oxford Structural Microscopy and Imaging Centre, University of Oxford, South Parks Road, Oxford OX1 3RE.
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Tobias Dietsche
4Section of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tübingen, Elfriede-Aulhorn-Str. 6, 72076 Tübingen, Germany
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Mehari Tesfazgi Mebrhatu
4Section of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tübingen, Elfriede-Aulhorn-Str. 6, 72076 Tübingen, Germany
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Tariq Ganief
5Proteome Center Tübingen, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen,Germany
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Boris Macek
5Proteome Center Tübingen, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen,Germany
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Samuel Wagner
4Section of Cellular and Molecular Microbiology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tübingen, Elfriede-Aulhorn-Str. 6, 72076 Tübingen, Germany
6German Center for Infection Research, Partner-site Tübingen, Elfriede-Aulhorn-Str. 6, 72076 Tübingen, Germany
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Carol V. Robinson
2Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK.
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Susan M. Lea
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
3Central Oxford Structural Microscopy and Imaging Centre, University of Oxford, South Parks Road, Oxford OX1 3RE.
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  • ORCID record for Susan M. Lea
  • For correspondence: susan.lea@path.ox.ac.uk
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Summary

Export of proteins through type three secretion systems is critical for bacterial motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP/FliQ/FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. We here present the structure of this complex at 4.2 Å by cryo-electron microscopy. None of the subunits adopt canonical integral membrane protein topologies and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems localize the export gate as a core component of the periplasmic portion of the machinery, and cross-linking experiments confirm this observation. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream.

One Sentence Summary The core of the T3SS export gate forms a supra-membrane helical assembly

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted January 17, 2018.
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Structure of the Core of the Type Three Secretion System Export Apparatus
Lucas Kuhlen, Patrizia Abrusci, Steven Johnson, Joseph Gault, Justin Deme, Joseph Caesar, Tobias Dietsche, Mehari Tesfazgi Mebrhatu, Tariq Ganief, Boris Macek, Samuel Wagner, Carol V. Robinson, Susan M. Lea
bioRxiv 249128; doi: https://doi.org/10.1101/249128
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Structure of the Core of the Type Three Secretion System Export Apparatus
Lucas Kuhlen, Patrizia Abrusci, Steven Johnson, Joseph Gault, Justin Deme, Joseph Caesar, Tobias Dietsche, Mehari Tesfazgi Mebrhatu, Tariq Ganief, Boris Macek, Samuel Wagner, Carol V. Robinson, Susan M. Lea
bioRxiv 249128; doi: https://doi.org/10.1101/249128

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