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Dynamic centriolar relocalization of Polo kinase and Centrobin in early mitosis primes centrosome asymmetry in fly neural stem cells

Emmanuel Gallaud, Anjana Ramdas Nair, Nicole Horsley, Arnaud Monnard, Priyanka Singh, Tri Thanh Pham, David Salvador Garcia, Alexia Ferrand, View ORCID ProfileClemens Cabernard
doi: https://doi.org/10.1101/249375
Emmanuel Gallaud
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
6Institute of Genetics and Development of Rennes, UMR6290 CNRS – Université Rennes 1, Campus Santé de Villejean, 2 avenue du Pr. Léon Bernard, 35043 Rennes Cedex, France.
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Anjana Ramdas Nair
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
3NYU Abu Dhabi, Saadiyat Campus, Abu Dhabi, United Arab Emirates
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Nicole Horsley
2Department of Biology, University of Washington, Life Science Building, Seattle, WA 98105, USA
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Arnaud Monnard
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
2Department of Biology, University of Washington, Life Science Building, Seattle, WA 98105, USA
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Priyanka Singh
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
4Department of Bioscience & Bioengineering, Indian Institute of Technology Jodhpur, NH 65, Nagour Road, Karwar, Jodhpur District, Rajasthan, India 342037.
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Tri Thanh Pham
2Department of Biology, University of Washington, Life Science Building, Seattle, WA 98105, USA
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David Salvador Garcia
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
5Division of Cell Biology, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
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Alexia Ferrand
1Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland
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Clemens Cabernard
2Department of Biology, University of Washington, Life Science Building, Seattle, WA 98105, USA
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  • ORCID record for Clemens Cabernard
  • For correspondence: ccabern@uw.edu
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Abstract

Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older ‘mother’ and a younger ‘daughter’ centriole. Stem cells either inherit the mother or daughter centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the dynamics and mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-Structured Illumination Microscopy (3D-SIM) and live cell imaging we show that in fly neural stem cells (neuroblasts) the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) dynamically relocalize from the mother to the daughter centriole during mitosis. This mechanism generates a centrosome, containing two molecularly distinct centrioles by telophase. Cnb’s timely relocalization is regulated by Polo-mediated phosphorylation whereas Polo’s daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.

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Posted June 19, 2019.
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Dynamic centriolar relocalization of Polo kinase and Centrobin in early mitosis primes centrosome asymmetry in fly neural stem cells
Emmanuel Gallaud, Anjana Ramdas Nair, Nicole Horsley, Arnaud Monnard, Priyanka Singh, Tri Thanh Pham, David Salvador Garcia, Alexia Ferrand, Clemens Cabernard
bioRxiv 249375; doi: https://doi.org/10.1101/249375
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Dynamic centriolar relocalization of Polo kinase and Centrobin in early mitosis primes centrosome asymmetry in fly neural stem cells
Emmanuel Gallaud, Anjana Ramdas Nair, Nicole Horsley, Arnaud Monnard, Priyanka Singh, Tri Thanh Pham, David Salvador Garcia, Alexia Ferrand, Clemens Cabernard
bioRxiv 249375; doi: https://doi.org/10.1101/249375

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