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Elongator is required for root stem cell maintenance by regulating SHORT ROOT transcription

Linlin Qi, Xiaoyue Zhang, Huawei Zhai, Jian Liu, Fangming Wu, Chuanyou Li, Qian Chen
doi: https://doi.org/10.1101/251603
Linlin Qi
2State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Xiaoyue Zhang
2State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3University of Chinese Academy of Sciences, Beijing 100049, China
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Huawei Zhai
2State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3University of Chinese Academy of Sciences, Beijing 100049, China
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Jian Liu
1State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China
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Fangming Wu
2State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Chuanyou Li
2State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3University of Chinese Academy of Sciences, Beijing 100049, China
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  • For correspondence: cyli@genetics.ac.cn chenqiangenetics@163.com
Qian Chen
1State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China
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  • For correspondence: cyli@genetics.ac.cn chenqiangenetics@163.com
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Abstract

SHORTROOT (SHR) is essential for stem cell maintenance and radial patterning in Arabidopsis thaliana roots, but how its expression is regulated is still unknown. Here, we report that Elongator regulates the transcription of SHR. The depletion of Elongator drastically reduced SHR expression and led to defective root stem cell maintenance and radial patterning. The importance of the nuclear localization of Elongator for its functioning, together with the insensitivity of the elp1 mutant to the transcription elongation inhibitor 6-azauracil and the direct interaction of the ELP4 subunit with the C-terminal domain of RNA polymerase II (RNAPII CTD), support the notion that Elongator plays important roles in transcription elongation. Indeed, we found that ELP3 associates with the pre-mRNA of SHR and that mutation of Elongator reduces the enrichment of RNAPII on the SHR gene body. Moreover, Elongator interacted in vivo with SUPPRESSOR OF Ty4 (SPT4), a well-established transcription elongation factor that was recruited to the SHR locus. Together, these results demonstrate that Elongator acts in concert with SPT4 to regulate the transcription of SHR.

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Posted January 22, 2018.
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Elongator is required for root stem cell maintenance by regulating SHORT ROOT transcription
Linlin Qi, Xiaoyue Zhang, Huawei Zhai, Jian Liu, Fangming Wu, Chuanyou Li, Qian Chen
bioRxiv 251603; doi: https://doi.org/10.1101/251603
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Elongator is required for root stem cell maintenance by regulating SHORT ROOT transcription
Linlin Qi, Xiaoyue Zhang, Huawei Zhai, Jian Liu, Fangming Wu, Chuanyou Li, Qian Chen
bioRxiv 251603; doi: https://doi.org/10.1101/251603

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