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Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity

David López-Escardó, Jordi Paps, Colomban de Vargas, Ramon Massana, Iñaki Ruiz-Trillo, Javier del Campo
doi: https://doi.org/10.1101/253146
David López-Escardó
1Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain.
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Jordi Paps
2School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK
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Colomban de Vargas
3CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Roscoff, France
4UPMC Univ. Paris 06, UMR 7144, Station Biologique de Roscoff, Roscoff, France
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Ramon Massana
5Department of Marine Biology and Oceanography, Institut de Ciències del Mar (CSIC), Barcelona, Catalonia, Spain
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Iñaki Ruiz-Trillo
1Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain.
6ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Catalonia, Spain
7Departament de Genètica, Microbiología i Estadística, Universitat de Barcelona, Barcelona, Catalonia, Spain
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  • For correspondence: jdelcampo@icm.csic.es inaki.ruiz@ibe.upf-csic.es
Javier del Campo
1Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain.
5Department of Marine Biology and Oceanography, Institut de Ciències del Mar (CSIC), Barcelona, Catalonia, Spain
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  • For correspondence: jdelcampo@icm.csic.es inaki.ruiz@ibe.upf-csic.es
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Abstract

Although animals are among the best studied organisms, we still lack a full description of their diversity, especially for microscopic taxa. This is partly due to the time-consuming and costly nature of surveying animal diversity through morphological and molecular studies of individual taxa. A powerful alternative is the use of high-throughput environmental sequencing, providing molecular data from all organisms sampled. We here address the unknown diversity of animal phyla in marine environments using an extensive dataset designed to assess eukaryotic ribosomal diversity among European coastal locations. A multi-phylum assessment of marine animal diversity that includes water column and sediments, oxic and anoxic environments, and both DNA and RNA templates, revealed a high percentage of novel 18S rRNA sequences in most phyla, suggesting that marine environments have not yet been fully sampled at a molecular level. This novelty is especially high among Platyhelminthes, Acoelomorpha, and Nematoda, which are well studied from a morphological perspective and abundant in benthic environments. We also identified based on molecular data a potentially novel group of widespread tunicates. Moreover, we recovered a high number of reads for Ctenophora and Cnidaria in the smaller fractions suggesting their gametes might play a greater ecological role than previously suspected.

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Posted January 25, 2018.
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Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
David López-Escardó, Jordi Paps, Colomban de Vargas, Ramon Massana, Iñaki Ruiz-Trillo, Javier del Campo
bioRxiv 253146; doi: https://doi.org/10.1101/253146
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Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity
David López-Escardó, Jordi Paps, Colomban de Vargas, Ramon Massana, Iñaki Ruiz-Trillo, Javier del Campo
bioRxiv 253146; doi: https://doi.org/10.1101/253146

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