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SlARF10, an auxin response factor, is required for chlorophyll and sugar accumulation during tomato fruit development

Lihua Mei, Yujin Yuan, Mengbo Wu, Zehao Gong, Qian Zhang, Fengqing Yang, Qiang Zhang, Yingqing Luo, Xin Xu, Wenfa Zhang, Mingjun Miao, Zhengguo Li, Wei Deng
doi: https://doi.org/10.1101/253237
Lihua Mei
2School of Life Science, Chongqing University, Chongqing 400044, China
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Yujin Yuan
2School of Life Science, Chongqing University, Chongqing 400044, China
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Mengbo Wu
2School of Life Science, Chongqing University, Chongqing 400044, China
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Zehao Gong
2School of Life Science, Chongqing University, Chongqing 400044, China
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Qian Zhang
3School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
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Fengqing Yang
3School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
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Qiang Zhang
2School of Life Science, Chongqing University, Chongqing 400044, China
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Yingqing Luo
2School of Life Science, Chongqing University, Chongqing 400044, China
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Xin Xu
2School of Life Science, Chongqing University, Chongqing 400044, China
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Wenfa Zhang
2School of Life Science, Chongqing University, Chongqing 400044, China
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Mingjun Miao
4Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
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Zhengguo Li
2School of Life Science, Chongqing University, Chongqing 400044, China
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Wei Deng
2School of Life Science, Chongqing University, Chongqing 400044, China
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  • For correspondence: dengwei1977@cqu.edu.cn
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Abstract

Tomato green fruits photosynthesis contributes to fruit growth and carbon economy. Tomato auxin response factor 10 (SlARF10) is one of the members of ARF family. Our results showed that SlARF10 locates in the nucleus and has no transcriptional activity. SlARF10 was expressed in various tomato tissues, but highly expressed in green fruit. Up-regulation of SlARF10 produced dark green phenotype of fruits, whereas down-regulation of SlARF10 had light green phenotype. Autofluorescence and chlorophyll content analysis confirmed the phenotypes, which indicated that SlARF10 plays an important role in chlorophyll accumulation in tomato fruits. Up-regulation of SlARF10 increased the photochemical potential in tomato leaves and fruits. Furthermore, the SlARF10 up-regulating lines displayed improved accumulation of starch in fruits, whereas SlARF10 suppressed lines had inhibited starch accumulation. Up-regulation of SlARF10 increased the expression of AGPases, the starch biosynthesis genes. SlARF10 up-regulating lines had increased accumulation of SlGLK1 and SlGLK2 transcripts in fruits. The promoter sequence of SlGLK1 gene had two conserved ARF binding sites. SlARF10 may regulate the expression of SlGLK1, thus controlling chlorophyll accumulation, photosynthesis rates and sugars synthesis in fruits. Our study provided more insight on the link between auxin signaling, chloroplastic activity and sugar metabolism during the development of tomato fruits.

ARFs
Auxin Response Factors
RNAi
RNA interference
GLK
GOLDEN2-LIKE
DET1/hp2
The DE-ETIOLATED 1
DDB1
UV-DAMAGED DNA-BINDING PROTEIN 1
KNOXs
Class I KNOTTED1-LIKE HOMEOBOX
GC-MS
Gas Chromatography-Mass Spectrometry
qRT-PCR
Quantitative real time PCR
TFs
Transcription factors
WT
Wild-type
MR
Middle region
DB domain
DNA binding domain
CTD
C-terminal interaction domain
AD
Transcriptional activators
RD
Transcriptional repressors
B3
N-terminal DNA-binding domain

Highlight SlARF10 played an important role in the chlorophyll accumulation and photosynthesis in tomato fruits. SlARF10 was involved in starch accumulation by controlling the expression of starch synthesis related enzyme genes. SlARF10 may regulate the expression of SlGLK1, thus controlling chlorophyll accumulation, photosynthesis rates and sugars synthesis in tomato fruits.

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Posted January 24, 2018.
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SlARF10, an auxin response factor, is required for chlorophyll and sugar accumulation during tomato fruit development
Lihua Mei, Yujin Yuan, Mengbo Wu, Zehao Gong, Qian Zhang, Fengqing Yang, Qiang Zhang, Yingqing Luo, Xin Xu, Wenfa Zhang, Mingjun Miao, Zhengguo Li, Wei Deng
bioRxiv 253237; doi: https://doi.org/10.1101/253237
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SlARF10, an auxin response factor, is required for chlorophyll and sugar accumulation during tomato fruit development
Lihua Mei, Yujin Yuan, Mengbo Wu, Zehao Gong, Qian Zhang, Fengqing Yang, Qiang Zhang, Yingqing Luo, Xin Xu, Wenfa Zhang, Mingjun Miao, Zhengguo Li, Wei Deng
bioRxiv 253237; doi: https://doi.org/10.1101/253237

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