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Distinct contributions of tensile and shear stress on E-cadherin levels during morphogenesis

View ORCID ProfileGirish R Kale, Xingbo Yang, Jean-Marc Philippe, Madhav Mani, Pierre-Francois Lenne, View ORCID ProfileThomas Lecuit
doi: https://doi.org/10.1101/255158
Girish R Kale
Aix Marseille Universite, CNRS, IBDM - UMR7288, 13009 Marseille, France;
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  • ORCID record for Girish R Kale
Xingbo Yang
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA;
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Jean-Marc Philippe
Aix Marseille Universite, CNRS, IBDM - UMR7288, 13009 Marseille, France;
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Madhav Mani
Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA;
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Pierre-Francois Lenne
Aix Marseille Universite, CNRS, IBDM - UMR7288, 13009 Marseille, France;
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Thomas Lecuit
College de France, 11 Place Marcelin Berthelot, 75005 Paris, France
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  • For correspondence: thomas.lecuit@univ-amu.fr
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Abstract

During epithelial morphogenesis, cell contacts (junctions) are constantly remodeled by mechanical forces that work against adhesive forces. E-cadherin complexes play a pivotal role in this process by providing persistent cell adhesion and by transmitting mechanical tension. In this context, it is unclear how mechanical forces affect E-cadherin adhesion and junction dynamics. During Drosophila embryo axis elongation, Myosin-II activity in the apico-medial and junctional cortex generates mechanical forces to drive junction remodeling. Here we report that the ratio between Vinculin and E-cadherin intensities acts as a ratiometric readout for these mechanical forces (load) at E-cadherin complexes. Medial Myosin-II loads E-cadherin complexes on all junctions, exerts tensile forces, and increases levels of E-cadherin. Junctional Myosin-II, on the other hand, biases the distribution of load between junctions of the same cell, exerts shear forces, and decreases the levels of E-cadherin. This work suggests distinct effects of tensile versus shear stresses on E-cadherin adhesion.

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  • This is a revised version. This is reformatted according to format instructions for Nature Communications. This version has both main and supplementary figures, unlike the previous upload that had separate supplementary figures.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 28, 2018.
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Distinct contributions of tensile and shear stress on E-cadherin levels during morphogenesis
Girish R Kale, Xingbo Yang, Jean-Marc Philippe, Madhav Mani, Pierre-Francois Lenne, Thomas Lecuit
bioRxiv 255158; doi: https://doi.org/10.1101/255158
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Distinct contributions of tensile and shear stress on E-cadherin levels during morphogenesis
Girish R Kale, Xingbo Yang, Jean-Marc Philippe, Madhav Mani, Pierre-Francois Lenne, Thomas Lecuit
bioRxiv 255158; doi: https://doi.org/10.1101/255158

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