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In vivo topology converts competition for cell-matrix adhesion into directional migration

Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, View ORCID ProfileEric Theveneau
doi: https://doi.org/10.1101/256255
Fernanda Bajanca
1Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
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Nadège Gouignard
1Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
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Charlotte Colle
2Cell and Developmental Biology Department, University College London, London, UK.
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Maddy Parsons
3Randall Division for Biophysics, King’s College London, London, UK.
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Roberto Mayor
2Cell and Developmental Biology Department, University College London, London, UK.
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Eric Theveneau
1Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
2Cell and Developmental Biology Department, University College London, London, UK.
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  • ORCID record for Eric Theveneau
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Abstract

When migrating in vivo, cells are exposed to numerous, and somewhat conflicting, signals: chemokines, repellents, extracellular matrix, growth factors. The roles of several of these molecules have been studied individually in vitro or in vivo but we have yet to understand how cells integrate them. To start addressing this question, we used the cephalic neural crest as a model system and looked at the roles of its best examples of positive and negative signals: stromal-cell derived factor 1 (Sdf1/Cxcl12) and class3-Semaphorins. Our results indicate that Sdf1 and Sema3A antagonistically control cell-matrix adhesion via opposite effects on Rac1 activity at the single cell level. Directional migration at the population level emerges as a result of global Semaphorin-dependent confinement and broad activation of adhesion by Sdf1 in the context of a biased Fibronectin distribution. These results indicate that uneven in vivo topology renders the need for precise distribution of secreted signals mostly dispensable.

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Posted January 31, 2018.
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In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
bioRxiv 256255; doi: https://doi.org/10.1101/256255
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In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
bioRxiv 256255; doi: https://doi.org/10.1101/256255

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