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Mitochondrial targeting of glycolysis in a major lineage of eukaryotes

Carolina Río Bártulos, Matthew B. Rogers, Tom A. Williams, Eleni Gentekaki, Henner Brinkmann, Rüdiger Cerff, Marie-Françoise Liaud, Adrian B. Hehl, Nigel R. Yarlett, Ansgar Gruber, Peter G. Kroth, View ORCID ProfileMark van der Giezen
doi: https://doi.org/10.1101/257790
Carolina Río Bártulos
1Institute of Genetics, University of Braunschweig, Spielmannstr. 7, D-38106 Braunschweig, Germany.
9Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany.
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Matthew B. Rogers
2Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.
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Tom A. Williams
3School of Biological Sciences, University of Bristol, BS81TH, United Kingdom.
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Eleni Gentekaki
4Dalhousie University, Department of Biochemistry and Molecular Biology, Halifax, Canada, B3H1X5.
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Henner Brinkmann
5Département de Biochimie, Université de Montréal C.P. 6128, Montréal, Quebec, Canada.
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Rüdiger Cerff
1Institute of Genetics, University of Braunschweig, Spielmannstr. 7, D-38106 Braunschweig, Germany.
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Marie-Françoise Liaud
1Institute of Genetics, University of Braunschweig, Spielmannstr. 7, D-38106 Braunschweig, Germany.
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Adrian B. Hehl
7Institute of Parasitology, University of Zürich, Switzerland.
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Nigel R. Yarlett
8Department of Chemistry and Physical Sciences, Pace University, New York, NY 10038, USA.
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Ansgar Gruber
9Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany.
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Peter G. Kroth
9Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany.
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Mark van der Giezen
2Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.
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  • ORCID record for Mark van der Giezen
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Abstract

Glycolysis is a major cytosolic catabolic pathway that provides ATP for many organisms1. Mitochondria play an even more important role in the provision of additional cellular ATP for eukaryotes2. Here, we show that in many stramenopiles, the C3 part of glycolysis is localised in mitochondria. We discovered genuine mitochondrial targeting signals on the six last enzymes of glycolysis. These targeting signals are recognised and sufficient to import GFP into mitochondria of a heterologous host. Analysis of eukaryotic genomes identified these targeting signals on many glycolytic C3 enzymes in a large group of eukaryotes found in the SAR supergroup3, in particular the stramenopiles. Stramenopiles, or heterokonts, are a large group of ecologically important eukaryotes that includes multi- and unicellular algae such as kelp and diatoms, but also economically important oomycete pathogens such as Phytophthora infestans. Confocal immunomicroscopy confirmed the mitochondrial location of glycolytic enzymes for the human parasite Blastocystis. Enzyme assays on cellular fractions confirmed the presence of the C3 part of glycolysis in Blastocystis mitochondria. These activities are sensitive to treatment with proteases and Triton X-100 but not proteases alone. Our work clearly shows that core cellular metabolism is more plastic than previously imagined and suggests new strategies to combat stramenopile pathogens such as the causative agent of late potato blight, P. infestans.

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Posted January 31, 2018.
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Mitochondrial targeting of glycolysis in a major lineage of eukaryotes
Carolina Río Bártulos, Matthew B. Rogers, Tom A. Williams, Eleni Gentekaki, Henner Brinkmann, Rüdiger Cerff, Marie-Françoise Liaud, Adrian B. Hehl, Nigel R. Yarlett, Ansgar Gruber, Peter G. Kroth, Mark van der Giezen
bioRxiv 257790; doi: https://doi.org/10.1101/257790
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Mitochondrial targeting of glycolysis in a major lineage of eukaryotes
Carolina Río Bártulos, Matthew B. Rogers, Tom A. Williams, Eleni Gentekaki, Henner Brinkmann, Rüdiger Cerff, Marie-Françoise Liaud, Adrian B. Hehl, Nigel R. Yarlett, Ansgar Gruber, Peter G. Kroth, Mark van der Giezen
bioRxiv 257790; doi: https://doi.org/10.1101/257790

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