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Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

View ORCID ProfileE Kague, P.E Witten, M Soenens, CL Campos, T Lubiana, S Fisher, C Hammond, Brown K Robson, MR Passos-Bueno, A Huysseune
doi: https://doi.org/10.1101/259085
E Kague
1Department of Physiology, Pharmacology and Neuroscience, University of Bristol, BS8 1TD, United Kingdom
2Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brasil
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P.E Witten
3Evolutionary Developmental Biology, Ghent University, Belgium
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M Soenens
3Evolutionary Developmental Biology, Ghent University, Belgium
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CL Campos
2Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brasil
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T Lubiana
2Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brasil
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S Fisher
4Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, United States
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C Hammond
1Department of Physiology, Pharmacology and Neuroscience, University of Bristol, BS8 1TD, United Kingdom
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Brown K Robson
5School of Archaeology and Anthropology, University of Bristol, United Kingdom
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MR Passos-Bueno
2Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brasil
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A Huysseune
3Evolutionary Developmental Biology, Ghent University, Belgium
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Summary

The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 02, 2018.
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Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth
E Kague, P.E Witten, M Soenens, CL Campos, T Lubiana, S Fisher, C Hammond, Brown K Robson, MR Passos-Bueno, A Huysseune
bioRxiv 259085; doi: https://doi.org/10.1101/259085
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Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth
E Kague, P.E Witten, M Soenens, CL Campos, T Lubiana, S Fisher, C Hammond, Brown K Robson, MR Passos-Bueno, A Huysseune
bioRxiv 259085; doi: https://doi.org/10.1101/259085

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