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Long-range Notch-mediated tissue patterning requires actomyosin contractility

View ORCID ProfileGinger L Hunter, Li He, Norbert Perrimon, Guillaume Charras, Edward Giniger, Buzz Baum
doi: https://doi.org/10.1101/259341
Ginger L Hunter
1National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA 20892
2MRC-LMCB, University College London, London, UK WC1E6BT
3Institute for the Physics of Living Systems, University College London, London, UK WC1E6BT
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  • ORCID record for Ginger L Hunter
Li He
4Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA, USA 02115
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Norbert Perrimon
4Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA, USA 02115
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Guillaume Charras
5London Centre for Nanotechnology, University College London, London, UK WC1E6BT
6Department of Cell and Developmental Biology, University College London, London, UK WC1E6BT
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Edward Giniger
1National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA 20892
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Buzz Baum
2MRC-LMCB, University College London, London, UK WC1E6BT
3Institute for the Physics of Living Systems, University College London, London, UK WC1E6BT
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Abstract

Dynamic, actin-based protrusions function in cell-cell signaling in a variety of systems. In the dorsal thorax of the developing fly, basal protrusions enable non-neighboring epithelial cells to touch, extending the range over which Notch-mediated lateral inhibition can occur during bristle patterning. Given that actin-based cell protrusions can exert mechanical forces on their environment and Notch receptor activation is mechanically sensitive, how might cytoskeletal contractility contribute to Notch signaling? We identify a pool of basal non-muscle myosin II (myosin II) that regulates protrusion dynamics, promotes Notch signaling, and is required in signal sending and receiving cells for Notch-dependent patterning. We show that interactions between protrusions are extensive and subject to actomyosin contractility. The effects of reducing myosin II activity are more pronounced for protrusion-mediated signaling than for signaling at lateral cell contacts. Together, these results reveal a role for actomyosin contractility in Notch activation, signaling, and patterning in a developmental context.

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Posted February 02, 2018.
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Long-range Notch-mediated tissue patterning requires actomyosin contractility
Ginger L Hunter, Li He, Norbert Perrimon, Guillaume Charras, Edward Giniger, Buzz Baum
bioRxiv 259341; doi: https://doi.org/10.1101/259341
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Long-range Notch-mediated tissue patterning requires actomyosin contractility
Ginger L Hunter, Li He, Norbert Perrimon, Guillaume Charras, Edward Giniger, Buzz Baum
bioRxiv 259341; doi: https://doi.org/10.1101/259341

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