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A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps

Thomas C. Terwilliger, Paul D. Adams, Pavel V. Afonine, Oleg V. Sobolev
doi: https://doi.org/10.1101/267138
Thomas C. Terwilliger
1Los Alamos National Laboratory, Los Alamos NM 87545 USA
2New Mexico Consortium, Los Alamos NM 87544 USA
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  • For correspondence: tterwilliger@newmexicoconsortium.org
Paul D. Adams
3Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8235, USA
4Department of Bioengineering, University of California Berkeley, Berkeley, CA, USA
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Pavel V. Afonine
3Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8235, USA
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Oleg V. Sobolev
3Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8235, USA
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Abstract

A fully automated procedure for optimization and interpretation of reconstructions from cryo-EM is developed and applied to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at lower resolution.

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Posted February 16, 2018.
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A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps
Thomas C. Terwilliger, Paul D. Adams, Pavel V. Afonine, Oleg V. Sobolev
bioRxiv 267138; doi: https://doi.org/10.1101/267138
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A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps
Thomas C. Terwilliger, Paul D. Adams, Pavel V. Afonine, Oleg V. Sobolev
bioRxiv 267138; doi: https://doi.org/10.1101/267138

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