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Elongation during segmentation shows axial variability, low mitotic rates, and synchronized cell cycle domains in the crustacean, Thamnocephalus platyurus

Savvas J Constantinou, Nicole Duan, Ariel D. Chipman, Lisa M. Nagy, View ORCID ProfileTerri A. Williams
doi: https://doi.org/10.1101/270728
Savvas J Constantinou
Biology Department, Trinity College, Hartford, CT, USA
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Nicole Duan
Biology Department, Trinity College, Hartford, CT, USA
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Ariel D. Chipman
The Department of Ecology, Evolution and Behavior, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram 91904, Jerusalem, Israel
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Lisa M. Nagy
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, 85721, USA
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Terri A. Williams
Biology Department, Trinity College, Hartford, CT, USA
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  • ORCID record for Terri A. Williams
  • For correspondence: terri.williams@trincoll.edu
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Abstract

Segmentation in arthropods typically occurs by sequential addition of segments from a posterior growth zone, but cell behaviors producing posterior elongation are not well known. Using precisely staged larvae of the crustacean, Thamnocephalus platyurus, we systematically examined cell division patterns and morphometric changes associated with posterior elongation during segmentation. We show that cell division is required for normal elongation but that cells in the growth zone need only divide ~1.5 times to meet that requirement; correspondingly, direct measures of cell division in the growth zone are low. Morphometric measurements of the growth zone and of newly formed segments suggest tagma-specific features of segment generation. Using methods for detecting two different phases in the cell cycle, we show distinct domains of synchronized cells in the posterior. Borders of cell cycle domains correlate with domains of segmental gene expression, suggesting an intimate link between segment generation and cell cycle regulation.

Summary Statement Posterior growth zone has synchronized cell cycle domains but shows little cell division during segment addition in a crustacean. Dimensions of the shrinking posterior growth zone change at tagma boundaries.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 23, 2018.
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Elongation during segmentation shows axial variability, low mitotic rates, and synchronized cell cycle domains in the crustacean, Thamnocephalus platyurus
Savvas J Constantinou, Nicole Duan, Ariel D. Chipman, Lisa M. Nagy, Terri A. Williams
bioRxiv 270728; doi: https://doi.org/10.1101/270728
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Elongation during segmentation shows axial variability, low mitotic rates, and synchronized cell cycle domains in the crustacean, Thamnocephalus platyurus
Savvas J Constantinou, Nicole Duan, Ariel D. Chipman, Lisa M. Nagy, Terri A. Williams
bioRxiv 270728; doi: https://doi.org/10.1101/270728

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