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α-proteobacterial RNA degradosomes assemble liquid-liquid phase separated RNP bodies

Nadra Al-Husini, Dylan T. Tomares, W. Seth Childers, View ORCID ProfileJared M. Schrader
doi: https://doi.org/10.1101/272286
Nadra Al-Husini
1Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA
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Dylan T. Tomares
2Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA
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W. Seth Childers
2Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA
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  • For correspondence: Schrader@wayne.edu wschild@pitt.edu
Jared M. Schrader
1Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA
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  • ORCID record for Jared M. Schrader
  • For correspondence: Schrader@wayne.edu wschild@pitt.edu
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Abstract

Ribonucleoprotein (RNP) granules play an important role in organizing eukaryotic mRNA metabolism via liquid-liquid phase separation (LLPS) of mRNA decay factors into membrane-less “droplet” organelles in the cytoplasm. Here we show that the bacterium Caulobacter crescentus Ribonuclease (RNase) E assembles RNP LLPS droplets that we term bacterial RNP-bodies (BR-bodies) similar to eukaryotic P-bodies and stress granules. RNase E requires RNA to assemble a BR-body, and disassembly requires RNA cleavage, suggesting BR-bodies provide localized sites of RNA degradation. The unstructured C-terminal domain of RNase E is both necessary and sufficient to assemble the core of the BR-body, is functionally conserved in related α-proteobacteria, and influences mRNA degradation. BR-bodies are rapidly induced under cellular stresses and provide enhanced cell growth under stress. To our knowledge, Caulobacter RNase E is the first bacterial protein identified that forms LLPS droplets, providing an effective strategy for subcellular organization in cells lacking membrane bound compartments.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 26, 2018.
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α-proteobacterial RNA degradosomes assemble liquid-liquid phase separated RNP bodies
Nadra Al-Husini, Dylan T. Tomares, W. Seth Childers, Jared M. Schrader
bioRxiv 272286; doi: https://doi.org/10.1101/272286
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α-proteobacterial RNA degradosomes assemble liquid-liquid phase separated RNP bodies
Nadra Al-Husini, Dylan T. Tomares, W. Seth Childers, Jared M. Schrader
bioRxiv 272286; doi: https://doi.org/10.1101/272286

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