Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Adaptive Reduction of Male Gamete Number in a Selfing Species

Takashi Tsuchimatsu, Hiroyuki Kakui, Misako Yamazaki, Cindy Marona, Hiroki Tsutsui, Afif Hedhly, Dazhe Meng, Yutaka Sato, Thomas Städler, Ueli Grossniklaus, Masahiro M. Kanaoka, Michael Lenhard, Magnus Nordborg, View ORCID ProfileKentaro K. Shimizu
doi: https://doi.org/10.1101/272757
Takashi Tsuchimatsu
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, 8057 Zurich, Switzerland
2Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland
3Gregor Mendel Institute, Austrian Academy of Sciences, A-1030 Vienna, Austria
4Department of Biology, Chiba University, Chiba 263-8522, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroyuki Kakui
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, 8057 Zurich, Switzerland
5Kihara Institute of Biological Research, Yokohama City University, Yokohama 244-0813, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Misako Yamazaki
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, 8057 Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cindy Marona
6Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroki Tsutsui
7Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan
8JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Nagoya 464-8602, Japan
9Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Afif Hedhly
2Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dazhe Meng
3Gregor Mendel Institute, Austrian Academy of Sciences, A-1030 Vienna, Austria
10Molecular and Computational Biology, University of Southern California, Los Angeles, California, 90089-0371 USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yutaka Sato
9Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Städler
11Institute of Integrative Biology, ETH Zurich, 8092 Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ueli Grossniklaus
2Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masahiro M. Kanaoka
7Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Lenhard
6Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Magnus Nordborg
3Gregor Mendel Institute, Austrian Academy of Sciences, A-1030 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kentaro K. Shimizu
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, 8057 Zurich, Switzerland
2Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland
5Kihara Institute of Biological Research, Yokohama City University, Yokohama 244-0813, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kentaro K. Shimizu
  • For correspondence: kentaro.shimizu@ieu.uzh.ch
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The number of male gametes produced is critical for reproductive success and varies greatly between and within species1–3. Evolutionary reduction of male gamete production has been widely reported in plants as a hallmark of the selfing syndrome, as well as in humans. Such a reduction may simply represent deleterious decay4–7, but evolutionary theory predicts that breeding systems could act as a major selective force on male gamete number: while large numbers of sperm should be produced in highly promiscuous species because of male–male gamete competition1, reduced sperm numbers may be advantageous at lower outcrossing rates because of the cost of gamete production. Here we used genome-wide association study (GWAS) to show a signature of polygenic selection on pollen number in the predominantly selfing plant Arabidopsis thaliana. The top associations with pollen number were significantly more strongly enriched for signatures of selection than those for ovule number and 107 phenotypes analyzed previously, indicating polygenic selection8. Underlying the strongest association, responsible for 20% of total pollen number variation, we identified the gene REDUCED POLLEN NUMBER 1 affecting cell proliferation in the male germ line. We validated its subtle but causal allelic effects using a quantitative complementation test with CRISPR-Cas9-generated null mutants in a nonstandard wild accession. Our results support polygenic adaptation underlying reduced male gamete numbers.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted February 28, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Adaptive Reduction of Male Gamete Number in a Selfing Species
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Adaptive Reduction of Male Gamete Number in a Selfing Species
Takashi Tsuchimatsu, Hiroyuki Kakui, Misako Yamazaki, Cindy Marona, Hiroki Tsutsui, Afif Hedhly, Dazhe Meng, Yutaka Sato, Thomas Städler, Ueli Grossniklaus, Masahiro M. Kanaoka, Michael Lenhard, Magnus Nordborg, Kentaro K. Shimizu
bioRxiv 272757; doi: https://doi.org/10.1101/272757
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Adaptive Reduction of Male Gamete Number in a Selfing Species
Takashi Tsuchimatsu, Hiroyuki Kakui, Misako Yamazaki, Cindy Marona, Hiroki Tsutsui, Afif Hedhly, Dazhe Meng, Yutaka Sato, Thomas Städler, Ueli Grossniklaus, Masahiro M. Kanaoka, Michael Lenhard, Magnus Nordborg, Kentaro K. Shimizu
bioRxiv 272757; doi: https://doi.org/10.1101/272757

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Evolutionary Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3482)
  • Biochemistry (7335)
  • Bioengineering (5305)
  • Bioinformatics (20217)
  • Biophysics (9988)
  • Cancer Biology (7712)
  • Cell Biology (11276)
  • Clinical Trials (138)
  • Developmental Biology (6426)
  • Ecology (9926)
  • Epidemiology (2065)
  • Evolutionary Biology (13294)
  • Genetics (9353)
  • Genomics (12564)
  • Immunology (7685)
  • Microbiology (18977)
  • Molecular Biology (7425)
  • Neuroscience (40931)
  • Paleontology (299)
  • Pathology (1226)
  • Pharmacology and Toxicology (2131)
  • Physiology (3145)
  • Plant Biology (6847)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1893)
  • Systems Biology (5306)
  • Zoology (1086)