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High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy

Bin Yang, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Bo Huang
doi: https://doi.org/10.1101/273359
Bin Yang
1Department of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA 94143, USA
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Yina Wang
1Department of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA 94143, USA
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Siyu Feng
2The UC Berkeley-UCSF Graduate Program in Bioengineering, San Francisco, CA 94143, USA
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Veronica Pessino
1Department of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA 94143, USA
3Graduate Program of Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
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Nico Stuurman
4Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94143, USA
5Howard Hughes Medical Institute, San Francisco, CA 94143 USA
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Bo Huang
1Department of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA 94143, USA
6Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
7Chan Zuckerberg Biohub, San Francisco, CA 94158, USA
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Abstract

Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for long-term volumetric imaging of living organisms. In order to achieve high-resolution imaging in common biological sample holders, we designed a high numerical aperture (NA) epi-illumination SPIM (eSPIM) system, which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. This system has an effective detection NA of > 1.06. We demonstrated multicolor and fast volumetric imaging of live cells and single-molecule super-resolution microscopy using our system.

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Posted February 28, 2018.
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High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy
Bin Yang, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Bo Huang
bioRxiv 273359; doi: https://doi.org/10.1101/273359
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High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy
Bin Yang, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Bo Huang
bioRxiv 273359; doi: https://doi.org/10.1101/273359

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