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Compartmentalized RNA catalysis in membrane - free coacervate protocells

Bjoern Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kresying, Hannes Mutschler, View ORCID ProfileT-Y. Dora Tang
doi: https://doi.org/10.1101/273417
Bjoern Drobot
Max-Planck Institute for Molecular Cell Biology and Genetics;
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Juan M. Iglesias-Artola
Max-Planck Institute for Molecular Cell Biology and Genetics;
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Kris Le Vay
Max-Planck Institute for Biochemistry
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Viktoria Mayr
Max-Planck Institute for Biochemistry
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Mrityunjoy Kar
Max-Planck Institute for Molecular Cell Biology and Genetics;
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Moritz Kresying
Max-Planck Institute for Molecular Cell Biology and Genetics;
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Hannes Mutschler
Max-Planck Institute for Biochemistry
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T-Y. Dora Tang
Max-Planck Institute for Molecular Cell Biology and Genetics;
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  • ORCID record for T-Y. Dora Tang
  • For correspondence: tang@mpi-cbg.de
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Abstract

Phase separation of mixtures of oppositely charged polymers provides a simple and direct route to compartmentalization via coacervation, which may have been important for driving primitive reactions as part of the RNA world hypothesis. However, to date, RNA catalysis has not been reconciled with coacervation. Here we demonstrate that RNA catalysis is viable within coacervate microdroplets and further show that these membrane-free droplets can selectively retain longer length RNAs while permitting transfer of lower molecular weight oligonucleotides.

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Posted February 28, 2018.
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Compartmentalized RNA catalysis in membrane - free coacervate protocells
Bjoern Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kresying, Hannes Mutschler, T-Y. Dora Tang
bioRxiv 273417; doi: https://doi.org/10.1101/273417
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Compartmentalized RNA catalysis in membrane - free coacervate protocells
Bjoern Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kresying, Hannes Mutschler, T-Y. Dora Tang
bioRxiv 273417; doi: https://doi.org/10.1101/273417

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