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Compartmentalized RNA catalysis in membrane - free coacervate protocells

Björn Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kreysing, Hannes Mutschler, View ORCID ProfileT-Y. Dora Tang
doi: https://doi.org/10.1101/273417
Björn Drobot
‡Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany
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Juan M. Iglesias-Artola
‡Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany
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Kris Le Vay
†Max-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
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Viktoria Mayr
†Max-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
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Mrityunjoy Kar
‡Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany
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Moritz Kreysing
‡Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany
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Hannes Mutschler
†Max-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
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T-Y. Dora Tang
‡Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307, Dresden, Germany
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  • ORCID record for T-Y. Dora Tang
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Abstract

Phase separation of mixtures of oppositely charged polymers provides a simple and direct route to compartmentalization via coacervation, which may have been important for driving primitive reactions as part of the RNA world hypothesis. However, to date, RNA catalysis has not been reconciled with coacervation. Here we demonstrate that RNA catalysis is viable within coacervate microdroplets and further show that these membrane-free droplets can selectively retain longer length RNAs while permitting transfer of lower molecular weight oligonucleotides.

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Posted February 28, 2018.
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Compartmentalized RNA catalysis in membrane - free coacervate protocells
Björn Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kreysing, Hannes Mutschler, T-Y. Dora Tang
bioRxiv 273417; doi: https://doi.org/10.1101/273417
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Compartmentalized RNA catalysis in membrane - free coacervate protocells
Björn Drobot, Juan M. Iglesias-Artola, Kris Le Vay, Viktoria Mayr, Mrityunjoy Kar, Moritz Kreysing, Hannes Mutschler, T-Y. Dora Tang
bioRxiv 273417; doi: https://doi.org/10.1101/273417

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