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Cryo-EM visualization of an active high open probability CFTR ion channel

View ORCID ProfileJonathan F. Fay, Luba A. Aleksandrov, Timothy J. Jensen, Liying L. Cui, Joseph N. Kousouros, Lihua He, Andrei A. Aleksandrov, Drew S. Gingerich, John R. Riordan, James Z. Chen
doi: https://doi.org/10.1101/274316
Jonathan F. Fay
1University of North Carolina, Chapel Hill
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Luba A. Aleksandrov
1University of North Carolina, Chapel Hill
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Timothy J. Jensen
1University of North Carolina, Chapel Hill
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Liying L. Cui
1University of North Carolina, Chapel Hill
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Joseph N. Kousouros
1University of North Carolina, Chapel Hill
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Lihua He
1University of North Carolina, Chapel Hill
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Andrei A. Aleksandrov
1University of North Carolina, Chapel Hill
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Drew S. Gingerich
2Oregon Health & Science University
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John R. Riordan
1University of North Carolina, Chapel Hill
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James Z. Chen
2Oregon Health & Science University
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Abstract

The Cystic fibrosis transmembrane conductance regulator (CFTR) anion channel, crucial to epithelial salt and water homeostasis, and defective due to mutations in its gene in patients with cystic fibrosis is a unique member of the large family of ATP-binding cassette transport proteins. Regulation of CFTR channel activity is stringently controlled by phosphorylation and nucleotide binding. Structural changes that underlie transitions between active and inactive functional states are not yet fully understood. Indeed the first 3D structures of dephosphorylated, ATP-free and phosphorylated ATP-bound states were only recently reported. Here we have determined the structure of inactive and active states of a thermally stabilized CFTR with very high channel open probability, confirmed after reconstitution into proteoliposomes. The unique repositioning of the TMHs and R domain density that we observe provide insights into the structural transition between active and inactive functional states of CFTR.

Highlights

  • Structures of thermostabilized avian CFTR in dephosphorylated or phosphorylated forms at 4.3 Å and 6.6 Å resolution, respectively.

  • Conformational differences of transmembrane helices 7 & 8 compared to zebra fish and human CFTR structures reveal an extracellular vestibule that may provide anion access to the pore.

  • R-domain density appears to “plug” the intercellular vestibule in the dephosphorylated avian CFTR cryo-EM map.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 02, 2018.
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Cryo-EM visualization of an active high open probability CFTR ion channel
Jonathan F. Fay, Luba A. Aleksandrov, Timothy J. Jensen, Liying L. Cui, Joseph N. Kousouros, Lihua He, Andrei A. Aleksandrov, Drew S. Gingerich, John R. Riordan, James Z. Chen
bioRxiv 274316; doi: https://doi.org/10.1101/274316
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Cryo-EM visualization of an active high open probability CFTR ion channel
Jonathan F. Fay, Luba A. Aleksandrov, Timothy J. Jensen, Liying L. Cui, Joseph N. Kousouros, Lihua He, Andrei A. Aleksandrov, Drew S. Gingerich, John R. Riordan, James Z. Chen
bioRxiv 274316; doi: https://doi.org/10.1101/274316

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