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Spatial organization of the somatosensory cortex revealed by cyclic smFISH

View ORCID ProfileSimone Codeluppi, View ORCID ProfileLars E. Borm, View ORCID ProfileAmit Zeisel, View ORCID ProfileGioele La Manno, Josina A. van Lunteren, View ORCID ProfileCamilla I. Svensson, View ORCID ProfileSten Linnarsson
doi: https://doi.org/10.1101/276097
Simone Codeluppi
1Division of molecular neurobiology, Department of medical biochemistry and biophysics, Karolinska institutet, Stockholm, Sweden
2Department of physiology and pharmacology, Karolinska institutet, Stockholm, Sweden
3Science for life laboratory, Stockholm, Sweden
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  • For correspondence: simone.codeluppi@ki.se sten.linnarsson@ki.se
Lars E. Borm
1Division of molecular neurobiology, Department of medical biochemistry and biophysics, Karolinska institutet, Stockholm, Sweden
3Science for life laboratory, Stockholm, Sweden
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Amit Zeisel
1Division of molecular neurobiology, Department of medical biochemistry and biophysics, Karolinska institutet, Stockholm, Sweden
3Science for life laboratory, Stockholm, Sweden
5Current address: Faculty of biotechnology and food engineering, Technion, Haifa, Israel
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Gioele La Manno
1Division of molecular neurobiology, Department of medical biochemistry and biophysics, Karolinska institutet, Stockholm, Sweden
3Science for life laboratory, Stockholm, Sweden
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Josina A. van Lunteren
4Department of biosciences and nutrition, Karolinska institutet, Stockholm, Sweden
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Camilla I. Svensson
2Department of physiology and pharmacology, Karolinska institutet, Stockholm, Sweden
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  • ORCID record for Camilla I. Svensson
Sten Linnarsson
1Division of molecular neurobiology, Department of medical biochemistry and biophysics, Karolinska institutet, Stockholm, Sweden
3Science for life laboratory, Stockholm, Sweden
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  • ORCID record for Sten Linnarsson
  • For correspondence: simone.codeluppi@ki.se sten.linnarsson@ki.se
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Abstract

The global efforts towards the creation of a molecular census of the brain using single-cell transcriptomics is generating a large catalog of molecularly defined cell types lacking spatial information. Thus, new methods are needed to map a large number of cell-specific markers simultaneously on large tissue areas. Here, we developed a cyclic single molecule fluorescence in situ hybridization methodology and defined the cellular organization of the somatosensory cortex using markers identified by single-cell transcriptomics.

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Posted March 04, 2018.
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Spatial organization of the somatosensory cortex revealed by cyclic smFISH
Simone Codeluppi, Lars E. Borm, Amit Zeisel, Gioele La Manno, Josina A. van Lunteren, Camilla I. Svensson, Sten Linnarsson
bioRxiv 276097; doi: https://doi.org/10.1101/276097
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Spatial organization of the somatosensory cortex revealed by cyclic smFISH
Simone Codeluppi, Lars E. Borm, Amit Zeisel, Gioele La Manno, Josina A. van Lunteren, Camilla I. Svensson, Sten Linnarsson
bioRxiv 276097; doi: https://doi.org/10.1101/276097

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