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Cryo-EM structure of alpha-synuclein fibrils

View ORCID ProfileRicardo Guerrero-Ferreira, Nicholas M. I. Taylor, Daniel Mona, Philippe Ringler, View ORCID ProfileMatthias E. Lauer, View ORCID ProfileRoland Riek, View ORCID ProfileMarkus Britschgi, Henning Stahlberg
doi: https://doi.org/10.1101/276436
Ricardo Guerrero-Ferreira
University of Basel;
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Nicholas M. I. Taylor
University of Basel;
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Daniel Mona
Hoffmann-La Roche;
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Philippe Ringler
University of Basel;
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Matthias E. Lauer
Hoffmann-La Roche;
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Roland Riek
ETH Zurich
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Markus Britschgi
Hoffmann-La Roche;
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Henning Stahlberg
University of Basel;
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  • For correspondence: henning.stahlberg@unibas.ch
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Abstract

Intracellular inclusions of alpha-synuclein are the neuropathological hallmark of progressive disorders called synucleinopathies. Alpha-synuclein fibrils are associated with transmissive cell-to-cell propagation of pathology. We report the structure of an alpha-synuclein fibril (residues 1-121) determined by cryo-electron microscopy at 3.4 Angstrom resolution. Two protofilaments form a polar fibril composed of staggered β-strands. The backbone of residues 38 to 95, including the fibril core and the non-amyloid component region, are well resolved in the EM map. Residues 50-57, containing three mutation sites associated with familial synucleinopathies, form the interface between the two protofilaments and contribute to fibril stability. A hydrophobic cleft may have implications for fibril elongation, and inform the rational design of molecules for diagnosis and treatment of synucleinopathies.

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Posted March 05, 2018.
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Cryo-EM structure of alpha-synuclein fibrils
Ricardo Guerrero-Ferreira, Nicholas M. I. Taylor, Daniel Mona, Philippe Ringler, Matthias E. Lauer, Roland Riek, Markus Britschgi, Henning Stahlberg
bioRxiv 276436; doi: https://doi.org/10.1101/276436
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Cryo-EM structure of alpha-synuclein fibrils
Ricardo Guerrero-Ferreira, Nicholas M. I. Taylor, Daniel Mona, Philippe Ringler, Matthias E. Lauer, Roland Riek, Markus Britschgi, Henning Stahlberg
bioRxiv 276436; doi: https://doi.org/10.1101/276436

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