Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Experimentally Induced Metamorphosis in Axolotl (Ambystoma mexicanum) Under Constant Diet Restructures Microbiota Accompanied by Reduced Limb Regenerative Capacity

Turan Demircan, Guvanch Ovezmyradov, Berna Yıldırım, İlknur Keskin, Ayse Elif İlhan, Ece Cana Fesçioğlu, Gürkan Öztürk, View ORCID ProfileSüleyman Yıldırım
doi: https://doi.org/10.1101/277285
Turan Demircan
1Department of Medical Biology, International School of Medicine, İstanbul Medipol University, Istanbul, Turkey
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guvanch Ovezmyradov
2Department of Biostatistics and Medical Informatics, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Berna Yıldırım
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
İlknur Keskin
3Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ayse Elif İlhan
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ece Cana Fesçioğlu
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gürkan Öztürk
4Department of Physiology, International School of Medicine, İstanbul Medipol University, Istanbul, Turkey
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Süleyman Yıldırım
5Department of Microbiology, International School of Medicine, İstanbul Medipol University, Istanbul, Turkey
6Regenerative and Restorative Medicine Research Center, REMER, İstanbul Medipol University, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Süleyman Yıldırım
  • For correspondence: suleymanyildirim@medipol.edu.tr tdemircan@medipol.edu.tr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Axolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite life-long neoteny in nature and in captive-bred colonies, metamorphosis of these animals can be experimentally induced by administering Thyroid hormones (THs). However, biological consequences of this experimental procedure, such as host microbiota response and implications for regenerative capacity, remain largely unknown. Here, we systematically compared host bacterial microbiota associated with skin, stomach, gut tissues and fecal samples based on 16S rRNA gene sequences, along with limb regenerative capacity, between neotenic and metamorphic Axolotls. Our results show that distinct bacterial communities inhabit individual organs of Axolotl and undergo substantial restructuring through metamorphosis. Drastic restructuring was observed for skin microbiota, highlighted by a major transition from Firmicutes-enriched to Proteobacteria-enriched relative abundance and precipitously decreased diversity. Remarkably, shifts in microbiota was accompanied by a steep reduction in limb regenerative capacity. Fecal microbiota of neotenic and metamorphic Axolotl shared relatively higher similarity, suggesting that diet continues to shape microbiota despite fundamental transformations in the host digestive organs. The results provide novel insights into microbiological and regenerative aspects of Axolotl metamorphosis and will establish a baseline for future in-depth studies.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 07, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Experimentally Induced Metamorphosis in Axolotl (Ambystoma mexicanum) Under Constant Diet Restructures Microbiota Accompanied by Reduced Limb Regenerative Capacity
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Experimentally Induced Metamorphosis in Axolotl (Ambystoma mexicanum) Under Constant Diet Restructures Microbiota Accompanied by Reduced Limb Regenerative Capacity
Turan Demircan, Guvanch Ovezmyradov, Berna Yıldırım, İlknur Keskin, Ayse Elif İlhan, Ece Cana Fesçioğlu, Gürkan Öztürk, Süleyman Yıldırım
bioRxiv 277285; doi: https://doi.org/10.1101/277285
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Experimentally Induced Metamorphosis in Axolotl (Ambystoma mexicanum) Under Constant Diet Restructures Microbiota Accompanied by Reduced Limb Regenerative Capacity
Turan Demircan, Guvanch Ovezmyradov, Berna Yıldırım, İlknur Keskin, Ayse Elif İlhan, Ece Cana Fesçioğlu, Gürkan Öztürk, Süleyman Yıldırım
bioRxiv 277285; doi: https://doi.org/10.1101/277285

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2409)
  • Biochemistry (4757)
  • Bioengineering (3300)
  • Bioinformatics (14584)
  • Biophysics (6591)
  • Cancer Biology (5132)
  • Cell Biology (7384)
  • Clinical Trials (138)
  • Developmental Biology (4327)
  • Ecology (6826)
  • Epidemiology (2057)
  • Evolutionary Biology (9843)
  • Genetics (7309)
  • Genomics (9471)
  • Immunology (4509)
  • Microbiology (12597)
  • Molecular Biology (4904)
  • Neuroscience (28113)
  • Paleontology (198)
  • Pathology (799)
  • Pharmacology and Toxicology (1372)
  • Physiology (1996)
  • Plant Biology (4452)
  • Scientific Communication and Education (970)
  • Synthetic Biology (1293)
  • Systems Biology (3894)
  • Zoology (718)