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An expression atlas of chemosensory ionotropic glutamate receptors identifies a molecular basis of carbonation detection

View ORCID ProfileJuan Antonio Sanchez-Alcaniz, Ana F Silbering, Vincent Croset, Giovanna Zappia, Anantha K Sivasubramaniam, Liliane Abuin, Saumya Y Sahai, Thomas O Auer, Steeve Cruchet, G. Larisa Neagu-Maier, View ORCID ProfileSimon G Sprecher, View ORCID ProfileNilay Yapici, View ORCID ProfileRichard Benton
doi: https://doi.org/10.1101/278804
Juan Antonio Sanchez-Alcaniz
University of Lausanne;
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Ana F Silbering
University of Lausanne;
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Vincent Croset
University of Lausanne;
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Giovanna Zappia
University of Lausanne;
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Anantha K Sivasubramaniam
University of Lausanne;
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Liliane Abuin
University of Lausanne;
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Saumya Y Sahai
Cornell University;
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Thomas O Auer
University of Lausanne;
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Steeve Cruchet
University of Lausanne;
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G. Larisa Neagu-Maier
University of Fribourg
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Simon G Sprecher
University of Fribourg
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Nilay Yapici
Cornell University;
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Richard Benton
University of Lausanne;
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  • For correspondence: richard.benton@unil.ch
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Abstract

Taste perception is thought to involve the encoding of appetitive and aversive chemical cues in food through a limited number of sensory pathways. Through expression analysis of the complete repertoire of Drosophila Ionotropic Receptors (IRs), a sensory subfamily of ionotropic glutamate receptors, we reveal that the majority of IRs is expressed in diverse peripheral neuron populations across gustatory organs in both larvae and adults, implying numerous roles in taste-evoked behaviours. We characterise Ir56d, which labels two anatomically-distinct classes of neurons in the proboscis: one represents a subset of sugar- and fatty acid-sensing neurons, while the other responds to carbonated solutions and fatty acids. Mutational analysis shows that IR56d, together with the broadly-expressed co-receptors IR25a and IR76b, is essential for physiological activation by carbonation and fatty acids, but not sucrose. We further demonstrate that carbonation is behaviourally attractive to flies (in an IR56d-dependent manner), but in a distinct way to other appetitive stimuli. Our work provides a valuable toolkit for investigating the taste functions of IRs, defines a molecular basis of carbonation sensing, and illustrates how the gustatory system uses combinatorial expression of sensory receptors in distinct neuron types to coordinate behaviour.

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Posted March 08, 2018.
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An expression atlas of chemosensory ionotropic glutamate receptors identifies a molecular basis of carbonation detection
Juan Antonio Sanchez-Alcaniz, Ana F Silbering, Vincent Croset, Giovanna Zappia, Anantha K Sivasubramaniam, Liliane Abuin, Saumya Y Sahai, Thomas O Auer, Steeve Cruchet, G. Larisa Neagu-Maier, Simon G Sprecher, Nilay Yapici, Richard Benton
bioRxiv 278804; doi: https://doi.org/10.1101/278804
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An expression atlas of chemosensory ionotropic glutamate receptors identifies a molecular basis of carbonation detection
Juan Antonio Sanchez-Alcaniz, Ana F Silbering, Vincent Croset, Giovanna Zappia, Anantha K Sivasubramaniam, Liliane Abuin, Saumya Y Sahai, Thomas O Auer, Steeve Cruchet, G. Larisa Neagu-Maier, Simon G Sprecher, Nilay Yapici, Richard Benton
bioRxiv 278804; doi: https://doi.org/10.1101/278804

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