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Effect of 5-trans isomer of arachidonic acid on model liposomal membranes studied by a combined simulation and experimental approach

Ioanna Tremi, View ORCID ProfileDimitrios Anagnostopoulos, View ORCID ProfileEllas Spyratou, View ORCID ProfileParaskevi Gkeka, View ORCID ProfileAlexandros G. Georgakilas, View ORCID ProfileChryssostomos Chatgilialoglu, View ORCID ProfileZoe Cournia
doi: https://doi.org/10.1101/279422
Ioanna Tremi
aDepartment of Physics, Technical University of Athens, Zografou Campus, 15773 Athens, Greece
bBiomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou, 11527 Athens, Greece
cInstitute of Nanoscience and Nanotechnology (INN), N.C.S.R. Demokritos, 10 Neapoleos, 15310 Athens, Greece
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Dimitrios Anagnostopoulos
cInstitute of Nanoscience and Nanotechnology (INN), N.C.S.R. Demokritos, 10 Neapoleos, 15310 Athens, Greece
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Ellas Spyratou
aDepartment of Physics, Technical University of Athens, Zografou Campus, 15773 Athens, Greece
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Paraskevi Gkeka
bBiomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou, 11527 Athens, Greece
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Alexandros G. Georgakilas
aDepartment of Physics, Technical University of Athens, Zografou Campus, 15773 Athens, Greece
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Chryssostomos Chatgilialoglu
cInstitute of Nanoscience and Nanotechnology (INN), N.C.S.R. Demokritos, 10 Neapoleos, 15310 Athens, Greece
dISOF, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy
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Zoe Cournia
bBiomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou, 11527 Athens, Greece
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  • ORCID record for Zoe Cournia
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Abstract

Unsaturated fatty acids are found in humans predominantly in the cis configuration. Fatty acids in the trans configuration are primarily the result of human processing (trans fats), but can also be formed endogenously by radical stress. The cis-trans isomerization of fatty acids by free radicals could be connected to several pathologies. Trans fats have been linked to an increased risk of coronary artery disease; however, the reasons for the resulting pathogenesis remain unclear. Here, we investigate the effect of a mono trans isomer of arachidonic acid (C20:4-5trans,8cis,11cis,14cis) produced by free radicals in physiological concentration on a model erythrocyte membrane using a combined experimental and theoretical approach. Molecular Dynamics (MD) simulations of two model lipid bilayers containing arachidonic acid and its 5-trans isomer in 3% mol. were carried out for this purpose. The 5-trans isomer formation in the phospholipids was catalyzed by HOCH2CH2S• radicals, generated from the corresponding thiol by γ-irradiation, in multilamellar vesicles (MLVs) of SAPC. Large unilamellar vesicles were made by the extrusion method (LUVET) as a biomimetic model for cis-trans isomerization. Atomic Force Microscopy and Dynamic Light Scattering were used to measure the average size, morphology, and the z-potential of the liposomes. Both results from MD simulations and experiments are in agreement and indicate that the two model membranes display different physicochemical properties in that the bilayers containing the trans fatty acids were more ordered and more rigid than those containing solely the cis arachidonic acid. Correspondingly, the average size of the liposomes containing trans isomers was smaller than the ones without.

Footnotes

  • E-mail: alexg{at}mail.ntua.gr; ioannatremi{at}mail.ntua.gr; ellas5{at}central.uoa.gr Tel: +30 2107723025

  • E-mail: zcournia{at}bioacademy.gr; Tel: +30 210 6597 195

  • E-mail: chrys{at}isof.cnr.it

  • dimitrios.x.anagnostopoulos{at}gsk.com

  • E-mail: Paraskevi.Gkeka{at}sanofi.com

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 09, 2018.
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Effect of 5-trans isomer of arachidonic acid on model liposomal membranes studied by a combined simulation and experimental approach
Ioanna Tremi, Dimitrios Anagnostopoulos, Ellas Spyratou, Paraskevi Gkeka, Alexandros G. Georgakilas, Chryssostomos Chatgilialoglu, Zoe Cournia
bioRxiv 279422; doi: https://doi.org/10.1101/279422
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Effect of 5-trans isomer of arachidonic acid on model liposomal membranes studied by a combined simulation and experimental approach
Ioanna Tremi, Dimitrios Anagnostopoulos, Ellas Spyratou, Paraskevi Gkeka, Alexandros G. Georgakilas, Chryssostomos Chatgilialoglu, Zoe Cournia
bioRxiv 279422; doi: https://doi.org/10.1101/279422

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