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CRISPR-Tag: an Efficient DNA Tagging System in Living Cells

Baohui Chen, Wei Zou, Bo Huang
doi: https://doi.org/10.1101/280495
Baohui Chen
1Department of Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
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  • For correspondence: bo.huang@ucsf.edu baohuichen@zju.edu.cn
Wei Zou
2The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, China
3Insititute of Translational Medicine, Zhejiang University, Hangzhou, China
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Bo Huang
4Department of Pharmaceutical Chemistry, University of California, San Francisco,San Francisco, California, USA.
5Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, USA.
6Chan Zuckerberg Biohub, San Francisco, California, USA.
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  • For correspondence: bo.huang@ucsf.edu baohuichen@zju.edu.cn
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A lack of efficient tools to image non-repetitive genes in living cells has limited our ability to explore the functional impact of spatiotemporal dynamics of genes. Here, we addressed this issue by developing the CRISPR-Tag system as a new DNA tagging strategy to label protein-coding genes with high signal-to-noise ratio under wild-field fluorescence microscopy by using 1 to 4 highly active sgRNAs. The CRISPR-Tag, with minimal size of ∼ 250 bp, represents an easily and broadly applicable technique to study spatiotemporal organization of genomic elements in living cells.

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Posted March 12, 2018.
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CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
Baohui Chen, Wei Zou, Bo Huang
bioRxiv 280495; doi: https://doi.org/10.1101/280495
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CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
Baohui Chen, Wei Zou, Bo Huang
bioRxiv 280495; doi: https://doi.org/10.1101/280495

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