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Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons

View ORCID ProfileJoseph D. Zak, Julien Grimaud, Rong-Chang Li, Chih-Chun Lin, Venkatesh N. Murthy
doi: https://doi.org/10.1101/282731
Joseph D. Zak
1Department of Molecular and Cellular Biology & Center for Brain Science, Harvard University, 16 Divinity Ave. Cambridge, MA 02138
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  • ORCID record for Joseph D. Zak
Julien Grimaud
1Department of Molecular and Cellular Biology & Center for Brain Science, Harvard University, 16 Divinity Ave. Cambridge, MA 02138
2Molecules, Cells & Organisms Program, Harvard University
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Rong-Chang Li
3Johns Hopkins University School of Medicine, Preclinical Teaching Building Room 905A, 725 North Wolfe Street Baltimore, MD 21205
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Chih-Chun Lin
3Johns Hopkins University School of Medicine, Preclinical Teaching Building Room 905A, 725 North Wolfe Street Baltimore, MD 21205
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Venkatesh N. Murthy
1Department of Molecular and Cellular Biology & Center for Brain Science, Harvard University, 16 Divinity Ave. Cambridge, MA 02138
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  • For correspondence: vnmurthy@fas.harvard.edu
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Abstract

The calcium-activated chloride channel anoctamin-2 (Ano2) is thought to amplify transduction currents in ORNs, a hypothesis supported by previous studies in dissociated neurons from Ano2-/- mice. Paradoxically, despite a reduction in transduction currents in Ano2-/- ORNs, their spike output for odor stimuli may be higher. We examined the role of Ano2 in ORNs in their native environment in freely breathing mice by imaging activity in ORN axons as they arrive in the olfactory bulb glomeruli. Odor-evoked responses in ORN axons of Ano2-/- mice were consistently larger for a variety of odorants and concentrations. In an open arena, Ano2-/- mice took longer to approach a localized odor source than wild-type mice, revealing clear olfactory behavioral deficits. Our studies provide the first in vivo evidence toward an alternative role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp ORN spike output.

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Posted March 15, 2018.
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Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons
Joseph D. Zak, Julien Grimaud, Rong-Chang Li, Chih-Chun Lin, Venkatesh N. Murthy
bioRxiv 282731; doi: https://doi.org/10.1101/282731
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Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons
Joseph D. Zak, Julien Grimaud, Rong-Chang Li, Chih-Chun Lin, Venkatesh N. Murthy
bioRxiv 282731; doi: https://doi.org/10.1101/282731

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