Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Clonal analysis reveals gradual recruitment of lateral root founder cells and a link between root initiation and cambium formation in Arabidopsis thaliana

View ORCID ProfileJoseph G. Dubrovsky
doi: https://doi.org/10.1101/283366
Joseph G. Dubrovsky
1Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Apartado Postal 510-3, Cuernavaca 62250, Mexico
2Department of Plant Sciences, University of Oxford, Oxford South Parks Road, Oxford OX1 3RB, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joseph G. Dubrovsky
  • For correspondence: jdubrov@ibt.unam.mx
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

  • The pericycle gives rise to lateral roots (LRs) and lateral meristems (LMs; cambium and phellogen), however, a thorough clonal analysis of pericycle cell lineage has not been investigated. This study fills in this gap and addresses pericycle impact in LR and LM development.

  • Heath-shock inducible DS1 transposition in 35S-DS1-H2B:YFP; HS-Ac seedlings results in production of YFP-labelled cell clones. These clones in pericycle cell derivatives were identified with a confocal microscopy and subjected to 3D reconstructions and analysis.

  • Participation of pericycle founder cells (FC) in LR formation is more variable than previously considered. LR initiation was found most commonly involved the specification of just one FC in the longitudinal and one or two cells in transverse direction. After LR initiation, FCs continue to be recruited in both directions from pre-existing cells. Anticlinal divisions in the pericycle resulting in LMs start already in the young differentiation zone where only the protoxylem is differentiated.

  • The clonal analysis demonstrated that pericycle cell activity related to LR formation is not separated in time and space from that related to LM formation and that LR FC recruitment is a gradual process. The analysis demonstrated that immediate pericycle progeny lack self-renewal capacity.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 17, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Clonal analysis reveals gradual recruitment of lateral root founder cells and a link between root initiation and cambium formation in Arabidopsis thaliana
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Clonal analysis reveals gradual recruitment of lateral root founder cells and a link between root initiation and cambium formation in Arabidopsis thaliana
Joseph G. Dubrovsky
bioRxiv 283366; doi: https://doi.org/10.1101/283366
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Clonal analysis reveals gradual recruitment of lateral root founder cells and a link between root initiation and cambium formation in Arabidopsis thaliana
Joseph G. Dubrovsky
bioRxiv 283366; doi: https://doi.org/10.1101/283366

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4089)
  • Biochemistry (8772)
  • Bioengineering (6487)
  • Bioinformatics (23356)
  • Biophysics (11756)
  • Cancer Biology (9154)
  • Cell Biology (13256)
  • Clinical Trials (138)
  • Developmental Biology (7417)
  • Ecology (11376)
  • Epidemiology (2066)
  • Evolutionary Biology (15094)
  • Genetics (10403)
  • Genomics (14014)
  • Immunology (9126)
  • Microbiology (22070)
  • Molecular Biology (8783)
  • Neuroscience (47393)
  • Paleontology (350)
  • Pathology (1421)
  • Pharmacology and Toxicology (2482)
  • Physiology (3705)
  • Plant Biology (8054)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2211)
  • Systems Biology (6017)
  • Zoology (1250)