ABSTRACT
ATP-sensitive potassium channels (KATP) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic KATP channels control insulin release and regulate metabolism at the whole body level. They are implicated in many metabolic disorders and diseases and are therefore important drug targets. Here, we present three structures of pancreatic KATP channels solved by cryo-electron microscopy (cryo-EM), at resolutions ranging from 4.1 to 4.5 Å. These structures depict the binding site of the antidiabetic drug glibenclamide, indicate how Kir6.2 N-terminus participates the coupling between the peripheral SUR1 subunit and the central Kir6.2 channel, reveal the binding mode of activating nucleotides, and suggest the mechanism of how Mg-ADP binding on nucleotide binding domains (NBDs) drives a conformational change of the SUR1 subunit.
ABBREVIATIONS
- KATP
- ATP-sensitive potassium channel
- cryo-EM
- cryo-electron microscopy
- SUR
- sulfonylurea receptor
- Kir6
- inward-rectifying potassium channel 6
- GBM
- glibenclamide
- ABC
- ATP-binding cassettes
- TMD0-L0
- transmembrane domain 0-loop 0
- NBD
- nucleotide binding domain
