Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Stress-Chip: A microfluidic platform for stress analysis in Caenorhabditis elegans

View ORCID ProfileStephen A. Banse, View ORCID ProfileBenjamin W. Blue, Kristin J. Robinson, Cody M. Jarrett, View ORCID ProfilePatrick C. Phillips
doi: https://doi.org/10.1101/285163
Stephen A. Banse
Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephen A. Banse
Benjamin W. Blue
Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benjamin W. Blue
Kristin J. Robinson
Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cody M. Jarrett
Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick C. Phillips
Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Patrick C. Phillips
  • For correspondence: pphil@uoregon.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

An organism’s ability to mount a physiological response to external stressors is fundamental to its interaction with the environment. Experimental exploration of these interactions benefits greatly from the ability to maintain tight control of the environment, even under conditions in which it would be normal for the subject to flee the stressor. ere we present a nematode research platform that pairs automated image acquisition and analysis with a custom microfluidic device. This platform enables tight environmental control in low-density, single-worm arenas, which preclude animal escape while still allowing a broad range of behavioral activities. The platform is easily scalable, with two 50 arena arrays per chip and an imaging capacity of 600 animals per scanning device. Validating the device using dietary, osmotic, and oxidative stress indicates that it should be of broad use as a research platform, including eventual adaptation for additional stressors, anthelmintic-drug screening, and toxicology studies.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 09, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Stress-Chip: A microfluidic platform for stress analysis in Caenorhabditis elegans
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Stress-Chip: A microfluidic platform for stress analysis in Caenorhabditis elegans
Stephen A. Banse, Benjamin W. Blue, Kristin J. Robinson, Cody M. Jarrett, Patrick C. Phillips
bioRxiv 285163; doi: https://doi.org/10.1101/285163
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The Stress-Chip: A microfluidic platform for stress analysis in Caenorhabditis elegans
Stephen A. Banse, Benjamin W. Blue, Kristin J. Robinson, Cody M. Jarrett, Patrick C. Phillips
bioRxiv 285163; doi: https://doi.org/10.1101/285163

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Physiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3686)
  • Biochemistry (7774)
  • Bioengineering (5668)
  • Bioinformatics (21244)
  • Biophysics (10563)
  • Cancer Biology (8160)
  • Cell Biology (11909)
  • Clinical Trials (138)
  • Developmental Biology (6738)
  • Ecology (10388)
  • Epidemiology (2065)
  • Evolutionary Biology (13843)
  • Genetics (9694)
  • Genomics (13056)
  • Immunology (8123)
  • Microbiology (19956)
  • Molecular Biology (7832)
  • Neuroscience (42971)
  • Paleontology (318)
  • Pathology (1276)
  • Pharmacology and Toxicology (2256)
  • Physiology (3350)
  • Plant Biology (7208)
  • Scientific Communication and Education (1309)
  • Synthetic Biology (1999)
  • Systems Biology (5528)
  • Zoology (1126)