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Sequential regulation of maternal mRNAs through a conserved cis-acting element in their 3’UTRs

Pooja Flora, Siu Wah Wong-Deyrup, Elliot Todd Martin, Ryan J Palumbo, Mohamad Nasrallah, Andrew Oligney, Patrick Blatt, Dhruv Patel, Gabriele Fuchs, Prashanth Rangan
doi: https://doi.org/10.1101/285569
Pooja Flora
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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Siu Wah Wong-Deyrup
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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Elliot Todd Martin
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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Ryan J Palumbo
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
#aCurrent address: Department of Biochemistry and Molecular Biology, Upstate Medical University, SUNY, Syracuse, NY 13210, USA
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Mohamad Nasrallah
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
#bCurrent address: University of Massachusetts Medical School, Worcester, MA 01605, USA
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Andrew Oligney
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
#cCurrent address: Touro College of Osteopathic Medicine (TouroCOM), Middletown, NY 10940
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Patrick Blatt
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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Dhruv Patel
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
#dCurrent address: Albany Medical College, Albany, NY 12208
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Gabriele Fuchs
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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Prashanth Rangan
1Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12222, USA
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  • For correspondence: prangan@albany.edu
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Abstract

Maternal mRNAs are synthesized during oogenesis to initiate the development of future generations. Some maternal mRNAs are determinants of somatic or germline fate and must be translationally repressed until embryogenesis. However, the translational repressors themselves are also temporally regulated. We use polar granule component (pgc), a Drosophila maternal mRNA, as a model system to ask how maternal mRNAs are repressed while the regulatory landscape is continually shifting. pgc, a potent transcriptional silencer and germline determinant, is translationally regulated throughout oogenesis. We find that the 3’UTR of pgc mRNA contains a conserved ten-nucleotide sequence that is bound by different conserved RNA binding proteins (RBPs) at different stages of oogenesis to continuously repress translation except for a brief expression in the stem cell daughter. Pumilio (Pum) binds to this sequence in undifferentiated and early differentiating oocytes and recruits other temporally restricted translational regulators to block pgc translation. After differentiation, Pum levels diminish and Bruno (Bru) levels increase, allowing Bru to bind the same 3’UTR sequence and take over translational repression of pgc mRNA. We have identified a class of maternal mRNAs regulated during oogenesis by both Pum and Bru, including Zelda, activator of the zygotic genome, which contain this core 10-nt regulatory sequence. Our data suggests that this hand off mechanism is more generally utilized to inhibit translation of maternal mRNAs during oogenesis.

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  • Competing interestsNo competing interests declared.

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Posted April 02, 2018.
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Sequential regulation of maternal mRNAs through a conserved cis-acting element in their 3’UTRs
Pooja Flora, Siu Wah Wong-Deyrup, Elliot Todd Martin, Ryan J Palumbo, Mohamad Nasrallah, Andrew Oligney, Patrick Blatt, Dhruv Patel, Gabriele Fuchs, Prashanth Rangan
bioRxiv 285569; doi: https://doi.org/10.1101/285569
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Sequential regulation of maternal mRNAs through a conserved cis-acting element in their 3’UTRs
Pooja Flora, Siu Wah Wong-Deyrup, Elliot Todd Martin, Ryan J Palumbo, Mohamad Nasrallah, Andrew Oligney, Patrick Blatt, Dhruv Patel, Gabriele Fuchs, Prashanth Rangan
bioRxiv 285569; doi: https://doi.org/10.1101/285569

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