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The Drosophila microbiome has a limited influence on sleep, activity, and courtship behaviors

View ORCID ProfileJoel Selkrig, View ORCID ProfileFarhan Mohammad, View ORCID ProfileSoon Hwee Ng, View ORCID ProfileChua Jia Yi, View ORCID ProfileTayfun Tumkaya, View ORCID ProfileJoses Ho, Yin Ning Chiang, View ORCID ProfileDirk Rieger, Sven Pettersson, View ORCID ProfileCharlotte Helfrich-Förster, View ORCID ProfileJoanne Y. Yew, View ORCID ProfileAdam Claridge-Chang
doi: https://doi.org/10.1101/287045
Joel Selkrig
1School of Biological Sciences; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
2European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
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Farhan Mohammad
3Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
4Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
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Soon Hwee Ng
5Temasek Life Sciences Laboratory; Department of Biological Science, National University of Singapore
6Centre for Evolutionary Biology, School of Animal Biology, University of Western Australia, Crawley, WA 6009, Australia
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Chua Jia Yi
3Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
4Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
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Tayfun Tumkaya
4Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
7Department of Physiology, National University of Singapore, Singapore
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Joses Ho
4Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
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Yin Ning Chiang
5Temasek Life Sciences Laboratory; Department of Biological Science, National University of Singapore
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Dirk Rieger
9Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany
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Sven Pettersson
1School of Biological Sciences; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
8Department of Microbiology, Cell and Tumor Biology, Karolinska Institutet, Solna, Sweden
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Charlotte Helfrich-Förster
9Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, 97074 Würzburg, Germany
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Joanne Y. Yew
5Temasek Life Sciences Laboratory; Department of Biological Science, National University of Singapore
10Pacific Biosciences Research Center, 1993 East West Road, University of Hawai’i at Mānoa, Honolulu, HI 96822, USA
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  • For correspondence: claridge-chang.adam@duke-nus.edu.sg jyew@hawaii.edu
Adam Claridge-Chang
3Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
4Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673
7Department of Physiology, National University of Singapore, Singapore
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  • For correspondence: claridge-chang.adam@duke-nus.edu.sg jyew@hawaii.edu
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Abstract

In animals, commensal microbes modulate various physiological functions, including behavior. While microbiota exposure is required for normal behavior in mammals, it is not known how widely this dependency is present in other animal species. We proposed the hypothesis that the microbiome has a major influence on the behavior of the vinegar fly (Drosophila melanogaster), a major invertebrate model organism. Several assays were used to test the contribution of the microbiome on some well-characterized behaviors: defensive behavior, sleep, locomotion, and courtship in microbe-bearing, control flies and two generations of germ-free animals. None of the behaviors were largely influenced by the absence of a microbiome, and the small or moderate effects were not generalizable between replicates and/or generations. These results refute the hypothesis, indicating that the Drosophila microbiome does not have a major influence over several behaviors fundamental to the animal’s survival and reproduction. The impact of commensal microbes on animal behaviour may not be broadly conserved.

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Posted March 22, 2018.
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The Drosophila microbiome has a limited influence on sleep, activity, and courtship behaviors
Joel Selkrig, Farhan Mohammad, Soon Hwee Ng, Chua Jia Yi, Tayfun Tumkaya, Joses Ho, Yin Ning Chiang, Dirk Rieger, Sven Pettersson, Charlotte Helfrich-Förster, Joanne Y. Yew, Adam Claridge-Chang
bioRxiv 287045; doi: https://doi.org/10.1101/287045
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The Drosophila microbiome has a limited influence on sleep, activity, and courtship behaviors
Joel Selkrig, Farhan Mohammad, Soon Hwee Ng, Chua Jia Yi, Tayfun Tumkaya, Joses Ho, Yin Ning Chiang, Dirk Rieger, Sven Pettersson, Charlotte Helfrich-Förster, Joanne Y. Yew, Adam Claridge-Chang
bioRxiv 287045; doi: https://doi.org/10.1101/287045

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