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Deep proteomic analysis of chicken erythropoiesis

Marjorie Leduc, Emilie-Fleur Gautier, Anissa Guillemin, Cédric Broussard, Virginie Salnot, Catherine Lacombe, Olivier Gandrillon, François Guillonneau, Patrick Mayeux
doi: https://doi.org/10.1101/289728
Marjorie Leduc
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, FranceLaboratory of Excellence GReX, F-75015 Paris, France
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Emilie-Fleur Gautier
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, FranceLaboratory of Excellence GReX, F-75015 Paris, France
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Anissa Guillemin
Université de Lyon, ENS de Lyon, Université Claude Bernard, CNRS UMR 5239, INSERM U1210, Laboratory of Biology and Modelling of the Cell, Lyon, France
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Cédric Broussard
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France
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Virginie Salnot
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France
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Catherine Lacombe
INSERM U1016, Institut Cochin, F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, FranceLaboratory of Excellence GReX, F-75015 Paris, France
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Olivier Gandrillon
Université de Lyon, ENS de Lyon, Université Claude Bernard, CNRS UMR 5239, INSERM U1210, Laboratory of Biology and Modelling of the Cell, Lyon, FranceInria Team Dracula, Inria Center Grenoble Rhône-Alpes, Lyon, France
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François Guillonneau
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France
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Patrick Mayeux
INSERM U1016, Institut Cochin, F-75014 Paris, FranceCNRS, UMR8104, F-75014 Paris, FrancePlateforme de Protéomique de l’Université Paris Descartes (3P5) F-75014 Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, FranceLaboratory of Excellence GReX, F-75015 Paris, France
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  • For correspondence: patrick.mayeux@inserm.fr
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Abstract

In contrast to mammalian erythroid cells that lost their nucleus at the end of the differentiation process, circulating chicken erythrocytes, like erythrocytes of most other non-mammalian vertebrates, are nucleated although their nucleus is believed to be transcriptionally silent. This major difference suggests that the erythroid differentiation process is likely to present both similarities and differences in mammals compared to other vertebrates. Since proteins are the major cellular effectors, analysis of the proteome is more prone to reflect true differences than analysis of the pattern of mRNA expression. We have previously reported the evolution of the proteome of human erythroid cells throughout their differentiation process. Here we report the analysis of the proteome of chicken erythroblasts during their terminal differentiation. We used the T2EC cellular model that allows to obtain homogenous populations of immature erythroblasts. Induction of their terminal differentiation led to their maturation and the possibility to obtain cells at different differentiation stages. Mass spectrometry analysis of these cell populations allowed the absolute quantification of 6167 proteins throughout the terminal differentiation process. Beside many proteins with similar expression patterns between chicken and human erythroblasts, like SLC4A1 (Band3), GATA1 or CD44, this analysis also revealed that other important proteins like Kit or other GATA transcription factors exhibit fully different patterns of expression.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 27, 2018.
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Deep proteomic analysis of chicken erythropoiesis
Marjorie Leduc, Emilie-Fleur Gautier, Anissa Guillemin, Cédric Broussard, Virginie Salnot, Catherine Lacombe, Olivier Gandrillon, François Guillonneau, Patrick Mayeux
bioRxiv 289728; doi: https://doi.org/10.1101/289728
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Deep proteomic analysis of chicken erythropoiesis
Marjorie Leduc, Emilie-Fleur Gautier, Anissa Guillemin, Cédric Broussard, Virginie Salnot, Catherine Lacombe, Olivier Gandrillon, François Guillonneau, Patrick Mayeux
bioRxiv 289728; doi: https://doi.org/10.1101/289728

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