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Changes in the genetic requirements for microbial interactions with increasing community complexity

Manon Morin, Emily C Pierce, View ORCID ProfileRachel J Dutton
doi: https://doi.org/10.1101/290353
Manon Morin
University of California, San Diego
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Emily C Pierce
University of California, San Diego
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Rachel J Dutton
University of California, San Diego
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  • ORCID record for Rachel J Dutton
  • For correspondence: rjdutton@ucsd.edu
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Abstract

Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in E. coli genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. E. coli genetic requirements varied among pairwise growth conditions and between pairwise and community conditions. Our analysis points to mechanisms by which growth conditions change as a result of increasing community complexity and suggests that growth within a community relies on a combination of pairwise and higher order interactions. Our work provides a framework for using the model organism E. coli as a probe to investigate microbial interactions regardless of the genetic tractability of members of the studied ecosystem.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 28, 2018.
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Changes in the genetic requirements for microbial interactions with increasing community complexity
Manon Morin, Emily C Pierce, Rachel J Dutton
bioRxiv 290353; doi: https://doi.org/10.1101/290353
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Changes in the genetic requirements for microbial interactions with increasing community complexity
Manon Morin, Emily C Pierce, Rachel J Dutton
bioRxiv 290353; doi: https://doi.org/10.1101/290353

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