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A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues

Celine Stoecklin, Zhang Yue, Wilhelm W. Chen, Richard de Mets, Eileen Fong, Vincent Studer, Virgile Viasnoff
doi: https://doi.org/10.1101/291104
Celine Stoecklin
1Mechanobiology Institute, 5A engineering drive 1, 117411 Singapore
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Zhang Yue
1Mechanobiology Institute, 5A engineering drive 1, 117411 Singapore
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Wilhelm W. Chen
1Mechanobiology Institute, 5A engineering drive 1, 117411 Singapore
2School of Materials Science and Engineering, Nanyang Technological University, N4.1, 50 Nanyang Avenue, Singapore 639798, Singapore
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Richard de Mets
1Mechanobiology Institute, 5A engineering drive 1, 117411 Singapore
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Eileen Fong
2School of Materials Science and Engineering, Nanyang Technological University, N4.1, 50 Nanyang Avenue, Singapore 639798, Singapore
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Vincent Studer
3CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France
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Virgile Viasnoff
1Mechanobiology Institute, 5A engineering drive 1, 117411 Singapore
4CNRS UMI 3639 5A engineering drive 1, 117411 Singapore
5Department of Biological Sciences, National university of Singapore, 14 Science Drive 4 Singapore 117543
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  • For correspondence: dbsvvnr@nus.edu.sg
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Abstract

The in vitro methods to recapitulate environmental cues around cells are usually optimized to test a specific property of the environment (biochemical nature or the stiffness of the extra cellular matrix (ECM), or nanotopography) for its capability to induce defined cell behaviors (lineage commitment, migration). Approaches that combine different environmental cues in 3D to assess the biological response of cells to the spatial organization of different biophysical and biochemical cues are growingly being developed. We demonstrate how the lamination of through-hole polymeric bio-functionalized membranes can be implemented to create complex bona fide micro-niches with differential 3D environmental properties using photoactive materials. Our approach enables to create micro-niches ranging in size from single cells to cell aggregates. They are bio-functionalized in 3D simultaneously with topographical featured, protein patterns and structured ECM surrogate with 1 micrometer resolution. We demonstrate how these niches extend in 3D the ability to pattern cells. We exemplify how they can be used to standardize cells shapes in 3D and to trigger the apico-basal polarization of single epithelial cells.

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Posted March 28, 2018.
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A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues
Celine Stoecklin, Zhang Yue, Wilhelm W. Chen, Richard de Mets, Eileen Fong, Vincent Studer, Virgile Viasnoff
bioRxiv 291104; doi: https://doi.org/10.1101/291104
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A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues
Celine Stoecklin, Zhang Yue, Wilhelm W. Chen, Richard de Mets, Eileen Fong, Vincent Studer, Virgile Viasnoff
bioRxiv 291104; doi: https://doi.org/10.1101/291104

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